Phosphotyrosine Eph receptor A4/SEK ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine Eph receptor A4/SEK ELISA Kit (AB279781)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine Eph receptor A4/SEK ELISA Kit (AB279781)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate for 10 mins at 37degC.
Reactivity data
Product details
Phospho-Eph receptor A4/SEK ELISA Kit (ab279781) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor A4/SEK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Eph receptor A4/SEK. An anti-Eph receptor A4/SEK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor A4/SEK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor A4/SEK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Biological function summary
EphA4 plays a critical role in developmental processes particularly in the development of the nervous system and vascular patterning. The receptor engages in bidirectional signaling meaning that signals are transmitted not only into the receptor-expressing cells but also into the ephrin-expressing ones. EphA4 is part of complexes that can include multiple receptor and ephrin molecules which allows for the modulation of cell adhesion and migration. These processes are essential for forming tissue boundaries and axon guidance.
Pathways
EphA4 signaling is involved in two significant pathways: the Eph/ephrin signaling pathway and the cytoskeletal remodeling pathway. In the Eph/ephrin signaling pathway EphA4 interacts with various ephrin ligands leading to changes in cellular architecture and behavior. The cytoskeletal remodeling pathway involves interactions with proteins such as Rho and Ras which coordinate cytoskeletal changes necessary for cell movement and positioning. These pathways are integral to processes like axon guidance and cell migration underpinning EphA4’s role in development.
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