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AB279782

Phosphotyrosine Eph receptor A5/BSK ELISA Kit

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Phosphotyrosine Eph receptor A5/BSK ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Eph receptor A5/BSK in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine Eph receptor A5/BSK ELISA Kit (AB279782)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine Eph receptor A5/BSK ELISA Kit (AB279782)

U251 cells untreated/treated with Pervanadate. U251 cells were untreated or treated with Pervanadate for 10 mins at 37degC.

Sandwich ELISA - Phosphotyrosine Eph receptor A5/BSK ELISA Kit (AB279782)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine Eph receptor A5/BSK ELISA Kit (AB279782)

Positive Control.

Jurkat cells were treated with Pervanadate at 37°C for 10 min.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-Eph receptor A5/BSK ELISA Kit (ab279782) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor A5/BSK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Eph receptor A5/BSK. An anti-Eph receptor A5/BSK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor A5/BSK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor A5/BSK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279782-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279782-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279782-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279782-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279782-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Eph receptor A5/BSK Coated Microplate", "number":"AB279782-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279782-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279782-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279782-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279782-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A5 also known as BSK is a member of the Eph family of receptor tyrosine kinases. It exhibits a molecular mass of approximately 116 kDa. This receptor is fundamentally involved in mediating cell adhesion and migration playing an essential role in cellular communication. Eph receptor A5/BSK is predominantly expressed in tissues of the nervous system including the brain and spinal cord which indicates an intrinsic role in neurodevelopment and synaptic organization.
Biological function summary

Eph receptor A5 engages in bidirectional signaling with its corresponding ephrin ligands creating a dynamic interaction network important for neuronal pathfinding and neurogenesis. This receptor acts as part of a larger complex self-associating with other family members to modulate cell position and boundary formation in development. The receptor's activity critically contributes to the regulation of axon guidance during neural development ensuring correct neuronal connections.

Pathways

Eph receptor A5 involves itself in the RhoA signaling pathway affecting cytoskeletal dynamics. It works alongside other Eph receptors and related proteins such as ephrin-A5 and Rho GTPases to manage cellular movement and growth cone stability. This receptor also integrates into the PI3K/AKT pathway influencing cell survival and proliferation where it potentially interfaces with insulin receptor substrates.

Eph receptor A5 shows connections to conditions like glioblastoma and Alzheimer's disease. In glioblastoma the aberrant signaling of Eph receptor A5 contributes to tumor progression and cell invasion interacting with proteins like EphA2. In Alzheimer's disease altered expression of this receptor affects synaptic function potentially leading to cognitive decline and it shares pathways with related proteins like beta-amyloid precursor protein.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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