JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279788

Phosphotyrosine ErbB2 / HER2 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phosphotyrosine ErbB2 / HER2 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine ErbB2 / HER2 in Human in Cell/Tissue Extracts samples.
2 Images
Sandwich ELISA - Phosphotyrosine ErbB2 / HER2 ELISA Kit (AB279788)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ErbB2 / HER2 ELISA Kit (AB279788)

Positive Control.

A431 cells were treated with EGF at 37°C for 20 min.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phosphotyrosine ErbB2 / HER2 ELISA Kit (AB279788)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ErbB2 / HER2 ELISA Kit (AB279788)

A431 cells treated with/without EGF. A431 cells were untreated or treated with EGF for 20 mins at 37degC.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phosphotyrosine ErbB2 / HER2 ELISA Kit (ab279788) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB2 / HER2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB2 / HER2. An anti-ErbB2 / HER2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated ErbB2 / HER2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ErbB2 / HER2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279788-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279788-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279788-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279788-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279788-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ErbB2 / HER2 Coated Microplate", "number":"AB279788-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - A431 cell lysate", "number":"AB279788-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279788-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279788-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279788-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB279787

Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit

Sandwich ELISA - Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit (AB279787)

  • 0
  • 0
Assay Kits

AB279773

Phosphotyrosine EGFR ELISA Kit

Sandwich ELISA - Phosphotyrosine EGFR ELISA Kit (AB279773)

  • 0
  • 0
Assay Kits

AB279792

Phosphotyrosine ErbB4 / HER4 ELISA Kit

Sandwich ELISA - Phosphotyrosine ErbB4 / HER4 ELISA Kit (AB279792)

  • 0
  • 0
Primary Antibodies

AB134182

Anti-ErbB2 / HER2 antibody [EP1045Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (AB134182)

  • 4
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com