Phosphotyrosine ErbB3 / HER3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine ErbB3 / HER3 in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phosphotyrosine ErbB3 / HER3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine ErbB3 / HER3 in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phosphotyrosine ErbB3 / HER3 ELISA Kit (ab279789) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB3 / HER3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB3 / HER3. An anti-pan ErbB3 / HER3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ErbB3 / HER3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated ErbB3 / HER3. After washing away unbound antibody, HRP conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ErbB3 / HER3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.
- Biological function summary
HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.
- Pathways
HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.
- Associated diseases and disorders
HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.
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2 product images
Sandwich ELISA - Phosphotyrosine ErbB3 / HER3 ELISA Kit (ab279789)
Positive Control.
A431 cells were treated with recombinant human EGF at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phosphotyrosine ErbB3 / HER3 ELISA Kit (ab279789)
A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 10 mins.
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