JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279792

Phosphotyrosine ErbB4 / HER4 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phosphotyrosine ErbB4 / HER4 ELISA Kit is a ELISA for the measurement of Phosphotyrosine ErbB4 / HER4 in Human in Cell/Tissue Extracts samples.
2 Images
Sandwich ELISA - Phosphotyrosine ErbB4 / HER4 ELISA Kit (AB279792)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ErbB4 / HER4 ELISA Kit (AB279792)

Positive Control.

T47D cells were treated with Pervanadate at 37°C for 10 mins.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA. .

Sandwich ELISA - Phosphotyrosine ErbB4 / HER4 ELISA Kit (AB279792)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ErbB4 / HER4 ELISA Kit (AB279792)

T47D cells treated/untreated with Pervanadate. T47D cells were treated or untreated with Pervanadate for 10 min at 37degC.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB5638

Anti-EGFR (phospho Y992) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y992) antibody (AB5638)

  • 0
  • 0
Primary Antibodies

AB109273

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)]

RabMAb|Recombinant

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)

  • 5
  • 1
Assay Kits

AB279788

Phosphotyrosine ErbB2 / HER2 ELISA Kit

Sandwich ELISA - Phosphotyrosine ErbB2 / HER2 ELISA Kit (AB279788)

  • 0
  • 0
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phosphotyrosine ErbB4 / HER4 ELISA Kit (ab279792) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB4 / HER4 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB4 / HER4. An anti-ErbB4 / HER4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated ErbB4 / HER4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ErbB4 / HER4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

For research use only - not intended for diagnostic use.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279792-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279792-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279792-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279792-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279792-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279792-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279792-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - T47D cell lysate", "number":"AB279792-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ErbB4 / HER4 Coated Microplate", "number":"AB279792-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279792-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ErbB4 also known as HER4 is a member of the ErbB family of receptor tyrosine kinases. It functions mechanically as a transmembrane protein that activates downstream signaling pathways upon ligand binding. This receptor has an approximate mass of 180 kDa. You can find ErbB4 mainly expressed in the heart brain and various epithelial tissues. The ErbB4 protein often interacts with ligands such as Neuregulins heregulin and others.
Biological function summary

ErbB4 has several roles including the regulation of cell proliferation differentiation migration and development. It functions as part of a receptor complex initiating a cascade of intracellular signaling that impacts various cellular functions. ErbB4 ELISA assays often help quantify interactions and activities of the receptor providing insights into its broader functionalities. The ErbB4 protein also contributes to neuronal development and the maintenance of cardiac tissue integrity.

Pathways

ErbB4 participates in the PI3K/AKT pathway and the MAPK/ERK pathway essential for cell survival and proliferation. These pathways involve a network of proteins including other ErbB family members such as EGFR ErbB2 (HER2) and ErbB3 (HER3) forming heterodimers that enhance and diversify the signaling outcomes. E200 and HF R are often referred to in literature related to pathway analysis of these complex interactions.

ErbB4 has links to several conditions notably certain cancers and schizophrenia. The connection to cancer arises due to its role in oncogenic signaling where it can form aberrant partnerships with proteins like ErbB2 which stimulates cell growth. In schizophrenia alterations in ErbB4 expression or function may affect neural signaling pathways leading to cognitive deficits. Understanding these relationships helps to target therapies and develop treatments involving anti HER HF R 500 and similar inhibitors.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com