Phosphotyrosine FGFR1 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine FGFR1 ELISA Kit (AB279797)
Positive Control.
K562 cells were treated with Pervanadate at 37°C for 10 mins.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine FGFR1 ELISA Kit (AB279797)
K562 cells untreated/treated with Pervanadate. K562 cells were treated or untreated with Pervanadate for 10 min at 37degC.
Reactivity data
Product details
Phosphotyrosine FGFR1 ELISA Kit (ab279797) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated FGFR1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-FGFR1. An anti-FGFR1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated FGFR1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect tyrosine-only phosphorylated FGFR1. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-FGFR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FGFR1 is significant in embryonic development and tissue repair. It does not function alone; rather it forms a complex with fibroblast growth factors and heparan sulfate proteoglycans facilitating receptor dimerization and autophosphorylation. FGFR1 is involved in bone growth angiogenesis and wound healing processes. The interaction of FGFR1 with these complex factors ensures the precise regulation of these critical physiological events in the body.
Pathways
FGFR1 participates actively in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are vital for transmitting signals from the cell surface to the DNA in the cell nucleus regulating gene expression and affecting cell cycle progression. FGFR1 works alongside similar proteins like FGFR2 influencing cell fate decisions. By modulating these pathways FGFR1 ensures a proper response to environmental signals which maintains homeostasis and adapts to physiological needs.
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