Skip to main content

Phosphotyrosine FGFR2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine FGFR2 in Human in Cell Lysate samples.

Be the first to review this product! Submit a review

Images

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
5X Assay Diluent
1 x 15 mL
600X HRP-Streptavidin Concentrate
1 x 200 µL
Biotinylated anti-phosphotyrosine antibody
2 x 1 Vial
Pan-FGFR2 Coated Microplate
1 x 1 Unit
Positive Control - treated Jurkat cell lysate
1 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Phosphotyrosine FGFR2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine FGFR2 in Human in Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phosphotyrosine FGFR2 ELISA Kit (ab279798) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated FGFR2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-FGFR2. An anti-FGFR2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FGFR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGFR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

For research use only - not intended for diagnostic use.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

Biological function summary

FGFR2 plays a role in diverse cellular processes like proliferation differentiation and migration. As part of a receptor-ligand complex FGFR2 partners with fibroblast growth factors to initiate signaling cascades. It contributes to embryonic development tissue repair and angiogenesis. FGFR2b expression predominantly appears in epithelial cells while FGFR2c is found in mesenchymal cells reflecting its distinct role in tissue-specific signaling.

Activity summary

The fibroblast growth factor receptor 2 (FGFR2) also known as CD332 is a protein that functions as a transmembrane receptor tyrosine kinase. FGFR2 is important in signal transduction where it binds to fibroblast growth factors triggering downstream intracellular pathways. FGFR2 has two main isoforms FGFR2b and FGFR2c created through alternative splicing. The molecular weight of FGFR2 varies by isoform typically around 100-110 kDa. It gets expressed in various tissues including epithelial and mesenchymal tissues.

Pathways

FGFR2 integrates into key signaling networks especially the MAPK and PI3K/AKT pathways. It interacts with proteins like FRS2 and GRB2 which facilitate the activation of downstream signals promoting cell survival and growth. FGFR2's engagement in these pathways is critical for maintaining normal cellular functions and contributes to its involvement in regulating complex cellular responses.

Associated diseases and disorders

FGFR2 mutations have connections with certain cancers and craniosynostosis syndromes. Alterations in FGFR2 signaling pathways can lead to aberrant cellular growth and have been found in various cancers including breast and gastric cancer. In craniosynostosis syndromes mutations in FGFR2 affect bone development linking it with disorders like Apert syndrome. These conditions demonstrate the pivotal role of FGFR2 in maintaining cellular homeostasis and the consequences of its dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Phosphotyrosine FGFR2 ELISA Kit (ab279798), expandable thumbnail

    Sandwich ELISA - Phosphotyrosine FGFR2 ELISA Kit (ab279798)

    Positive Control.

    Jurkat cells were treated with Pervanadate at 37°C for 10 min.

    Solubilize cells at 4 x 107 cells/ml in lysis buffer.

    Serial dilutions of lysates were analyzed in this ELISA.

  • Sandwich ELISA - Phosphotyrosine FGFR2 ELISA Kit (ab279798), expandable thumbnail

    Sandwich ELISA - Phosphotyrosine FGFR2 ELISA Kit (ab279798)

    Jurkat cells treated with/without Pervanadate. Jurkat cells were treated or untreated with Pervanadate for 10 mins at 37degC.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com