Phosphotyrosine FRK ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine FRK ELISA Kit (AB279803)
K562 cells untreated/treated with Pervanadate. K562 cells were untreated or treated with Pervanadate for 10 min at 37degC.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine FRK ELISA Kit (AB279803)
Positive Control.
K562 cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Reactivity data
Product details
Phosphotyrosine FRK ELISA Kit (ab279803) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated FRK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-FRK. An anti-pan FRK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FRK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated FRK. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
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Biological function summary
This kinase contributes to the regulation of cell cycle progression and apoptosis. FRK acts solo as well as part of larger signal transduction complexes. It plays a vital role in limiting uncontrolled cellular growth which is particularly significant in maintaining tissue homeostasis. FRK regulates various molecular interactions essential for cell adhesion and communication adding another layer of control over cell physiology.
Pathways
FRK integrates into signaling pathways like the PI3K/AKT and MAPK pathways. These pathways are critical for mediating responses to growth signals and stress conditions. FRK's activity modulates protein interactions with key molecules such as p53 and PTEN influencing downstream effects that control cell cycle checkpoints and apoptosis. By interacting with these pathways FRK helps to balance cell proliferation and survival in response to extracellular cues.
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