Phosphotyrosine Fyn ELISA Kit
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- sELISA
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Sandwich ELISA - Phosphotyrosine Fyn ELISA Kit (AB279807)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate for 10 mins at 37degC.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine Fyn ELISA Kit (AB279807)
Jurkat cells were treated with Pervanadate at 37°C for 10 mins.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
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Product details
Phosphotyrosine Fyn ELISA Kit (ab279807) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Fyn protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Fyn. An anti-pan Fyn antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Fyn present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect tyrosine-phosphorylated protein. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phosphorylated Fyn bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
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Biological function summary
Fyn kinase influences cell growth differentiation and survival. It is a part of larger signaling complexes including those accountable for the development and function of the central nervous system. The protein's activity is important for neural plasticity and supports synaptic communication particularly within the brain. Fyn can interact with other proteins in these complexes to help direct cellular responses to external signals.
Pathways
Fyn kinase is central to several intracellular signaling pathways such as the T cell receptor (TCR) signaling pathway and the integrin signaling pathway. The TCR signaling pathway involves the regulation of immune responses while the integrin signaling pathway contributes to cell adhesion and migration. Other proteins like Lck and Yes interact with Fyn within these pathways adding another layer of regulation and response in various cell types.
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