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AB279858

Phosphotyrosine GM-CSF Receptor alpha ELISA Kit

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Phosphotyrosine GM-CSF Receptor alpha ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine GM-CSF Receptor alpha in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine GM-CSF Receptor alpha ELISA Kit (AB279858)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine GM-CSF Receptor alpha ELISA Kit (AB279858)

THP-1 cells untreated/treated with Pervanadate.

THP1 cells were untreated or treated with Pervanadate for 10 minutes at 37°C.

Sandwich ELISA - Phosphotyrosine GM-CSF Receptor alpha ELISA Kit (AB279858)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine GM-CSF Receptor alpha ELISA Kit (AB279858)

Positive Control.

Jurkat cells were treated with Pervanadate at 37°C for 10 min.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphotyrosine GM-CSF Receptor alpha ELISA Kit (ab279858) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated GM-CSF Receptor alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-GM-CSF Receptor alpha. An anti-pan GM-CSF Receptor alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated GM-CSF Receptor alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-GM-CSF Receptor alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279858-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279858-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279858-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279858-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279858-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-GM-CSF Receptor alpha Coated Microplate", "number":"AB279858-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279858-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279858-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279858-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279858-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GM-CSF receptor alpha also known as CD116 is a protein that plays role in the immune response. It is a component of the GM-CSF receptor complex and has an approximate molecular weight of 74 kDa. This receptor is found on the surface of various cell types like monocytes macrophages and neutrophils. It binds specifically to granulocyte-macrophage colony-stimulating factor (GM-CSF) which leads to the activation of downstream signaling pathways important for cell survival and proliferation.
Biological function summary

GM-CSF receptor alpha binds GM-CSF with high affinity and is part of a receptor complex that includes beta common chain (βc). The receptor complex initiates signaling cascades that modulate immune functions such as differentiation of myeloid cells and macrophage activation. These interactions support various immune processes like inflammation and pathogen defense. GM-CSF receptor alpha is essential to ensure proper cellular responses to environmental cues.

Pathways

GM-CSF receptor alpha is involved in the JAK-STAT and MAPK pathways. Upon ligand binding it activates JAK2 which in turn triggers STAT5 phosphorylation — important for transcription of immune-related genes. Simultaneously it initiates the MAPK (mitogen-activated protein kinase) pathway further promoting cell differentiation and proliferation. Each of these signaling events connect GM-CSF receptor alpha to other proteins like JAK2 and STAT5 which collaborate to sustain proper immune activity.

GM-CSF receptor alpha is linked to pulmonary alveolar proteinosis and rheumatoid arthritis. In pulmonary alveolar proteinosis dysregulation of GM-CSF receptor signaling leads to impaired surfactant clearance resulting in respiratory complications. In rheumatoid arthritis increased GM-CSF signaling contributes to inflammation and joint damage. Proteins like βc and GM-CSF interact with GM-CSF receptor alpha amplifying the pathological signals in these conditions highlighting its potential as a therapeutic target.
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Product protocols

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Target data

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websiteProtocolBooklet
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