Phosphotyrosine In-Cell ELISA Kit
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Phosphotyrosine In-Cell ELISA Kit is a Cell-based (qualitative) ELISA for the measurement of Phosphotyrosine In-Cell in Human, Mouse, Rat in Cell Samples samples.
View Alternative Names
L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine
- In-Cell ELISA
Unknown
In-Cell ELISA - Phosphotyrosine In-Cell ELISA Kit (AB126431)
A431 cells were treated for 30 min with 50 μL of 100 mM Genistein or 2 mM Lavendustin in appropriate wells at room temperature prior to EGF stimulation. Added 50 μL different concentrations of rhEGF (0, 20 or 100 ng/ml in serum free DMEM) to appropriate wells. Then incubated for 10 min at 37°C.
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Product details
ab126431 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Phosphotyrosine and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining Phosphotyrosine protein in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the Cell-Based Phosphotyrosine ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, HRP-Anti- Phosphotyrosine is pipetted into the wells and incubated. The wells are washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
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