Phosphotyrosine iNOS ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine iNOS ELISA Kit (AB279829)
THP-1 cells untreated/treated with Pervanadate.
THP1 cells were untreated or treated with Pervanadate for 10 mins at 37°C.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine iNOS ELISA Kit (AB279829)
Positive Control.
THP1 cells were treated with Pervanadate.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Reactivity data
Product details
Phosphotyrosine iNOS ELISA Kit (ab279829) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated iNOS protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-iNOS. An anti-iNOS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated iNOS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-iNOS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
Pathways
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
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