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AB279835

Phosphotyrosine ITK/EMT ELISA Kit

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Phosphotyrosine ITK/EMT ELISA Kit is a ELISA for the measurement of Phosphotyrosine ITK/EMT in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine ITK/EMT ELISA Kit (AB279835)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ITK/EMT ELISA Kit (AB279835)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were treated with Pervanadate at 37°C for 10 mins.

Sandwich ELISA - Phosphotyrosine ITK/EMT ELISA Kit (AB279835)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine ITK/EMT ELISA Kit (AB279835)

Positive Control.

Jurkat cells were treated with Pervanadate at 37°C for 10 mins.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphotyrosine ITK/EMT ELISA Kit (ab279835) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ITK/EMT protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ITK/EMT. An anti-pan ITK/EMT antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated ITK/EMT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ITK/EMT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279835-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279835-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279835-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279835-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279835-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279835-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ITK/EMT Coated Microplate", "number":"AB279835-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279835-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279835-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279835-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITK also known as Interleukin-2-inducible T-cell kinase is a protein belonging to the Tec family of non-receptor tyrosine kinases. It weighs approximately 72 kDa and plays a significant role in signal transduction in immune cells specifically T cells. ITK is widely expressed in lymphoid tissues particularly in thymocytes and peripheral T cells. ITK products often include variants like ITK-1 and ITK kit which are useful in various scientific applications.
Biological function summary

ITK is integral to the activation and development of T cells which are critical components of the adaptive immune response. ITK functions as part of a larger signaling complex interacting with proteins such as LAT and SLP-76 to mediate downstream signaling events following T-cell receptor engagement. This interaction facilitates the phosphorylation of multiple substrates which is necessary for T-cell proliferation differentiation and cytokine production.

Pathways

ITK plays an important role in T-cell receptor signaling and the downstream Ras/MAPK pathway. It interacts with proteins like ZAP-70 and LCK in these pathways which are important for initiating T-cell activation and function. The modulation of these pathways by ITK is essential for proper immune function and response to antigens.

ITK is linked to immunodeficiency and autoimmune disorders. Dysregulation of ITK activity can lead to conditions like X-linked lymphoproliferative syndrome and systemic lupus erythematosus. The EMT protein also ties with ITK concerning certain neoplastic conditions where aberrant signaling contributes to the pathogenesis. Researchers study ITK's interactions with other proteins such as BSA EMT to understand its role in these diseases better and explore potential therapeutic targets.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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