Phosphotyrosine LTK ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine LTK ELISA Kit (AB279854)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine LTK ELISA Kit (AB279854)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were untreated or treated with Pervanadate for 10 minutes at 37°C.
Reactivity data
Product details
Phosphotyrosine LTK ELISA Kit (ab279854) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated LTK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-LTK. An anti-pan LTK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated LTK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-LTK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The LTK protein functions in the differentiation and development of cells particularly in the nervous and immune systems. It oligomerizes forming homodimers or heterodimers which activates its kinase activity. This activation promotes phosphorylation events that influence cellular processes like growth and survival. The receptor is not part of a larger complex but interacts directly with several adaptor proteins to exert its effects.
Pathways
LTK is involved in the MAPK/ERK and PI3K/AKT pathways important for cell growth and survival signaling. The MAPK/ERK pathway includes related proteins such as RAS and RAF while the PI3K/AKT pathway interacts with proteins like PIK3CA and AKT1. These pathways converge on transcription factors and regulators that ultimately coordinate cellular responses to external signals.
Product protocols
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Target data
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Product promise
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