Phosphotyrosine PDGFR alpha ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine PDGFR alpha ELISA Kit (AB279884)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were untreated or treated with Pervanadate for 10 minutes at 37°C.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine PDGFR alpha ELISA Kit (AB279884)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
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Reactivity data
Product details
Phosphotyrosine PDGFR alpha ELISA Kit (ab279884) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PDGFR alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-PDGFR alpha. An anti-PDGFR alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated PDGFR alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-PDGFR alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
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Biological function summary
PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.
Pathways
PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.
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