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AB279885

Phosphotyrosine PDGFR beta ELISA Kit

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Phosphotyrosine PDGFR beta ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine PDGFR beta in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine PDGFR beta ELISA Kit (AB279885)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine PDGFR beta ELISA Kit (AB279885)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were untreated or treated with Pervanadate for 10 minutes at 37°C.

Sandwich ELISA - Phosphotyrosine PDGFR beta ELISA Kit (AB279885)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine PDGFR beta ELISA Kit (AB279885)

Positive Control.

Jurkat cells were treated with Pervanadate at 37°C for 10 min.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphotyrosine PDGFR beta ELISA Kit (ab279885) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PDGFR beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-PDGFR beta. An anti-PDGFR beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated PDGFR beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-PDGFR beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279885-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279885-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279885-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279885-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279885-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-PDGFR beta Coated Microplate", "number":"AB279885-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279885-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279885-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279885-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279885-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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