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AB279942

Phosphotyrosine STAT4 ELISA Kit

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Phosphotyrosine STAT4 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine STAT4 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine STAT4 ELISA Kit (AB279942)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine STAT4 ELISA Kit (AB279942)

HeLa cells treated with/without pervanadate.

HeLa cells were untreated or treated with Pervanadate for 10 min at 37°C.

Sandwich ELISA - Phosphotyrosine STAT4 ELISA Kit (AB279942)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine STAT4 ELISA Kit (AB279942)

Positive Control.

HeLa cells were treated with Pervanadate at 37°C for 10 min.

Cells were solubilzed at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphotyrosine STAT4 ELISA Kit (ab279942) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated STAT4 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-STAT4. An anti-STAT4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated STAT4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-STAT4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279942-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279942-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279942-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279942-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-STAT4 Coated Microplate", "number":"AB279942-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279942-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279942-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279942-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279942-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279942-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Signal Transducer and Activator of Transcription 4 (STAT4) is a critical member of the STAT protein family. It weighs approximately 85 kDa. STAT4 primarily functions as a transcription factor activated by phosphorylation through Janus kinases (JAKs) in response to cytokine signaling. This protein facilitates the translocation into the nucleus where it promotes the transcription of specific genes. STAT4 expression occurs mainly in T cells and natural killer (NK) cells indicating its role in the immune response.
Biological function summary

STAT4 is integral to the differentiation of naïve T cells into T helper 1 (Th1) cells. These Th1 cells play a central role in orchestrating immune responses against intracellular pathogens. STAT4 activation requires two distinct steps involving JAK kinases and subsequent binding to DNA regulatory elements of target genes. STAT4 does not function as part of a large protein complex but rather operates as a dimer following phosphorylation.

Pathways

STAT4 participates strongly in the JAK-STAT signaling pathway fundamental for immune cell maturation and response. The JAK-STAT pathway regulates several biological processes including proliferation and inflammation. STAT4 works closely with STAT1 in promoting Th1 differentiation while also interacting with other proteins like IL-12 to modulate immune activity. These interactions emphasize its influence in immune responses and could affect inflammatory pathways.

STAT4 exhibits associations with autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant STAT4 activity can lead to dysregulated immune responses making it a significant target in these disorders. In the context of rheumatoid arthritis altered STAT4 signaling enhances inflammation through its connection with other immune proteins like IL-23. Understanding STAT4's role in these diseases may offer avenues for therapeutic interventions and better management of immune-related conditions.
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Product protocols

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Target data

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websiteProtocolBooklet
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