Phosphotyrosine Syk ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine Syk ELISA Kit (AB279949)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine Syk ELISA Kit (AB279949)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were untreated or treated with Pervanadate for 10 min at 37°C.
Reactivity data
Product details
Phosphotyrosine Syk ELISA Kit (ab279949) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Syk protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Syk. An anti-Syk antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Syk present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiphosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Syk bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Syk plays a role in transmitting signals from the cell surface to the interior of the cell facilitating various cellular activities. It is a part of signal transduction complexes and functions in the regulation of immune cell activation differentiation and survival. It affects processes like Fc receptor signaling in macrophages and neutrophils impacting immune responses and inflammation. The phospho-Syk form influences the strength and duration of signaling affecting various cellular functions.
Pathways
Syk is significant in the B-cell receptor (BCR) signaling pathway and Fc receptor signaling pathway. Within these pathways Syk interacts with proteins like Lyn and Fyn which are other kinases involved in BCR signal transduction. Syk phosphorylation noted as p-Syk plays a part in activating downstream molecules such as phospholipase C gamma (PLCγ) to mediate cellular responses to external stimuli. The critical role of Syk in these pathways influences both adaptive and innate immune responses.
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