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AB279960

Phosphotyrosine TXK/RLX ELISA Kit

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Phosphotyrosine TXK/RLX ELISA Kit is a ELISA for the measurement of Phosphotyrosine TXK/RLX in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phosphotyrosine TXK/RLX ELISA Kit (AB279960)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine TXK/RLX ELISA Kit (AB279960)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were untreated or treated with Pervanadate for 10 min at 37°C.

Sandwich ELISA - Phosphotyrosine TXK/RLX ELISA Kit (AB279960)
  • sELISA

Supplier Data

Sandwich ELISA - Phosphotyrosine TXK/RLX ELISA Kit (AB279960)

Positive Control.

Jurkat cells were treated with Pervanadate at 37°C for 10 min.

Solubilize cells at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphotyrosine TXK/RLX ELISA Kit (ab279960) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TXK/RLX protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TXK/RLX. An anti- TXK/RLX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated TXK/RLX present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho- TXK/RLX bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279960-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279960-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated Jurkat cell lysate", "number":"AB279960-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phosphotyrosine antibody", "number":"AB279960-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-TXK/RLX Coated Microplate", "number":"AB279960-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279960-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279960-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279960-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279960-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279960-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXK also known as RLK is a member of the Tec family of non-receptor tyrosine kinases. It has a molecular mass of approximately 62 kDa. This protein is mainly expressed in the cytoplasm and nucleus of T-cells and natural killer cells. Its structure includes an SH3 domain SH2 domain and a catalytic domain enabling its role as a kinase. TXK contributes to cell signaling by phosphorylating downstream proteins following receptor stimulation.
Biological function summary

The TXK protein functions in the regulation of immune cell activation and differentiation. It interacts with other molecules in the cell to form signaling complexes. In T cells TXK aids in the secretion of cytokines contributing to the immune response. The protein also engages with various cellular pathways to influence transcription factor activity affecting gene expression related to immune function.

Pathways

TXK significantly impacts the T-cell receptor signaling pathway and natural killer cell activation pathway. Its activity links with other proteins such as LCK and FYN which are also involved in the modulation of immune responses. Through these pathways TXK plays a role in regulating cellular responses to external stimuli ultimately affecting immune system function and activity.

TXK has associations with autoimmune diseases and certain types of cancer. In autoimmune conditions such as rheumatoid arthritis abnormal TXK signaling may result in inappropriate immune activation. Furthermore its connections with proteins like FYN highlight TXK's potential involvement in leukemia where dysregulated signaling pathways contribute to uncontrolled cell proliferation. Understanding TXK's roles in these diseases may help in developing targeted therapeutic strategies.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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