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Phosphotyrosine VEGF Receptor 3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine VEGF Receptor 3 in Human in Cell Lysate samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
300X Streptavidin-Conjugated HRP
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Biotinylated anti-phosphotyrosine antibody
2 x 1 Vial
Pan-VEGF Receptor 3 Coated Microplate
1 x 1 Unit
Positive Control - K562 cell lysate
1 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Phosphotyrosine VEGF Receptor 3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine VEGF Receptor 3 in Human in Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated VEGF Receptor 3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-VEGF Receptor 3. An anti-VEGF Receptor 3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated VEGF Receptor 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-VEGF Receptor 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

Biological function summary

The VEGF Receptor 3 acts as an important regulator of lymphangiogenesis which is the growth of new lymphatic vessels from pre-existing ones. It is not typically part of larger protein complexes but it works closely with its ligands to facilitate the processes associated with lymph vessel formation and function. Proper activity of VEGF Receptor 3 is necessary for maintaining normal lymphatic vessel remodeling and function during development and in some adult tissues.

Activity summary

VEGF Receptor 3 also known as FLT-4 is a protein essential for lymphatic development and is expressed mainly in lymphatic endothelial cells. This receptor has a molecular mass of approximately 195 kDa. VEGF Receptor 3 belongs to the tyrosine kinase family and binds with high affinity to its ligands VEGF-C and VEGF-D. These interactions lead to the activation of intracellular signaling cascades that promote cell proliferation migration and survival.

Pathways

The receptor is involved in the VEGF signaling pathway which plays an important role in vascular and lymphatic development. This pathway intersects with others like the PI3K/AKT pathway a critical mediator of cell growth and survival. VEGF Receptor 3 interacts with related receptors such as VEGF Receptor 2 in these pathways to regulate complex and essential physiological processes.

Associated diseases and disorders

VEGF Receptor 3 has been linked to lymphedema which is swelling due to lymphatic system dysfunction and certain cancers where excessive angiogenesis occurs. Mutations or dysregulations in the receptor's signaling can lead to these conditions. In cancer for instance VEGF Receptor 3 along with its associated protein VEGF-C can promote tumor lymphangiogenesis facilitating metastasis. Antagonists like anti-VEGF therapies target these receptors to inhibit tumor progression and manage these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967), expandable thumbnail

    Sandwich ELISA - Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967)

    Positive Control.

    K562 cells were treated with Pervanadate at 37°C for 10 min.

    Solubilize cells at 4 x 107 cells/ml in lysis buffer.

    Serial dilutions of lysates were analyzed in this ELISA.

  • Sandwich ELISA - Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967), expandable thumbnail

    Sandwich ELISA - Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967)

    HUVEC cells treated/untreated with pervanadate.

    HUVEC cells were untreated or treated with Pervanadate for 10 min at 37°C.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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