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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Phosphotyrosine VEGF Receptor 3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine VEGF Receptor 3 in Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Phosphotyrosine VEGF Receptor 3 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosphotyrosine VEGF Receptor 3 in Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
Pre-coated microplate (12 x 8 well strips)
Blue Ice
-20°C
-20°C
-20°C
Phosphotyrosine VEGF Receptor 3 ELISA Kit (ab279967) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated VEGF Receptor 3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-VEGF Receptor 3. An anti-VEGF Receptor 3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated VEGF Receptor 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-VEGF Receptor 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
The VEGF Receptor 3 acts as an important regulator of lymphangiogenesis which is the growth of new lymphatic vessels from pre-existing ones. It is not typically part of larger protein complexes but it works closely with its ligands to facilitate the processes associated with lymph vessel formation and function. Proper activity of VEGF Receptor 3 is necessary for maintaining normal lymphatic vessel remodeling and function during development and in some adult tissues.
VEGF Receptor 3 also known as FLT-4 is a protein essential for lymphatic development and is expressed mainly in lymphatic endothelial cells. This receptor has a molecular mass of approximately 195 kDa. VEGF Receptor 3 belongs to the tyrosine kinase family and binds with high affinity to its ligands VEGF-C and VEGF-D. These interactions lead to the activation of intracellular signaling cascades that promote cell proliferation migration and survival.
The receptor is involved in the VEGF signaling pathway which plays an important role in vascular and lymphatic development. This pathway intersects with others like the PI3K/AKT pathway a critical mediator of cell growth and survival. VEGF Receptor 3 interacts with related receptors such as VEGF Receptor 2 in these pathways to regulate complex and essential physiological processes.
VEGF Receptor 3 has been linked to lymphedema which is swelling due to lymphatic system dysfunction and certain cancers where excessive angiogenesis occurs. Mutations or dysregulations in the receptor's signaling can lead to these conditions. In cancer for instance VEGF Receptor 3 along with its associated protein VEGF-C can promote tumor lymphangiogenesis facilitating metastasis. Antagonists like anti-VEGF therapies target these receptors to inhibit tumor progression and manage these disorders.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Positive Control.
K562 cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
HUVEC cells treated/untreated with pervanadate.
HUVEC cells were untreated or treated with Pervanadate for 10 min at 37°C.
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