Pig Fibrinogen ELISA Kit

Product details

Pig Fibrinogen ELISA Kit ab205090 is a sandwich ELISA to measure Pig Fibrinogen in serum, citrate plasma, EDTA plasma, heparin plasma with a sensitivity of 1.713 ng/mL

How the assay works:

In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Pig Fibrinogen ELISA Kit ab205090 protocol summary

1. Anti-FIB Antibodies Bound To Solid Phase
2. Standards and Samples Added
3. FIB * Anti-FIB Complexes Formed
4. Unbound Sample Proteins Removed
5. Anti-FIB-HRP Conjugate Added
6. Anti-FIB-HRP * FIB * Anti-FIB Complexes Formed
7. Unbound Anti-FIB-HRP Removed
8. Chromogenic Substrate Added
9. Determine Bound Enzyme Activity

In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.