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AB205091

Pig Haptoglobin ELISA Kit

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(5 Publications)

Pig Haptoglobin ELISA Kit is a sandwich ELISA designed to quantify Pig Haptoglobin with a sensitivity of 3.107 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 400 ng/mL

View Alternative Names

Haptoglobin, HP

1 Images
Sandwich ELISA - Pig Haptoglobin ELISA Kit (AB205091)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Haptoglobin ELISA Kit (AB205091)

Representative standard curve using ab205091 Pig Haptoglobin ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.107 ng/mL

Range

6.25 - 400 ng/mL

Assay time

40m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Pig Haptoglobin (ab205091) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological fluid of pigs.

In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205091-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Pig Hapatoglobin Calibrator (lyophilized)", "number":"AB205091-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentration", "number":"AB205091-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205091-CMP05", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Conjugated Enzyme Antibody", "number":"AB205091-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205091-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205091-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Pig Haptoglobin ELISA Microplate", "number":"AB205091-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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Product protocols

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Target data

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
See full target information HP
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 12: PubMed39591145

2024

Well-Being and Performance of Nursery Pigs Subjected to Different Commercial Vaccines Against Porcine Circovirus Type 2, and .

Applications

Unspecified application

Species

Unspecified reactive species

Caio Abércio Silva,Marco Aurélio Callegari,Cleandro Pazinato Dias,Kelly Lais de Souza,Gabrieli Souza Romano,Luciana Fiorin Hernig,Ricardo Tesche Lippke,Rutger Jansen,Fernando Lopes Leite,Fernando Filipe,Rafael Humberto de Carvalho

Journal of animal science and biotechnology 14:158 PubMed38143275

2023

Effects of early postnatal gastric and colonic microbiota transplantation on piglet gut health.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Larsen,Simone Margaard Offersen,Anders Brunse,Mattia Pirolo,Soumya Kanti Kar,Luca Guadabassi,Thomas Thymann

Biomedicines 10: PubMed36289758

2022

Model of Acute Liver Failure in an Isolated Perfused Porcine Liver-Challenges and Lessons Learned.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Hefler,Sanaz Hatami,Aducio Thiesen,Carly Olafson,Kiarra Durand,Jason Acker,Constantine J Karvellas,David L Bigam,Darren H Freed,Andrew Mark James Shapiro

Animals : an open access journal from MDPI 11: PubMed34438871

2021

Meloxicam and Dexamethasone Administration as Anti-Inflammatory Compounds to Sows Prior to Farrowing Does Not Improve Lactation Performance.

Applications

Unspecified application

Species

Unspecified reactive species

Kate J Plush,John R Pluske,David S Lines,Cameron R Ralph,Roy N Kirkwood

Animals : an open access journal from MDPI 11: PubMed34070802

2021

Positive Human Contact and Housing Systems Impact the Responses of Piglets to Various Stressors.

Applications

Unspecified application

Species

Unspecified reactive species

Megan E Hayes,Lauren M Hemsworth,Rebecca S Morrison,Alan J Tilbrook,Paul H Hemsworth
View all publications
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Product promise

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