Pig Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Pig Haptoglobin in Pig in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
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- Journal of animal science and biotechnology 14:1582023Effects of early postnatal gastric and colonic microbiota transplantation on piglet gut health.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristina Larsen,Simone Margaard Offersen,Anders Brunse,Mattia Pirolo,Soumya Kanti Kar,Luca Guadabassi,Thomas ThymannPubMed 38143275
- Biomedicines 10:2022Model of Acute Liver Failure in an Isolated Perfused Porcine Liver-Challenges and Lessons Learned.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoshua Hefler,Sanaz Hatami,Aducio Thiesen,Carly Olafson,Kiarra Durand,Jason Acker,Constantine J Karvellas,David L Bigam,Darren H Freed,Andrew Mark James ShapiroPubMed 36289758
- Animals : an open access journal from MDPI 11:2021Meloxicam and Dexamethasone Administration as Anti-Inflammatory Compounds to Sows Prior to Farrowing Does Not Improve Lactation Performance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKate J Plush,John R Pluske,David S Lines,Cameron R Ralph,Roy N KirkwoodPubMed 34438871
- Animals : an open access journal from MDPI 11:2021Positive Human Contact and Housing Systems Impact the Responses of Piglets to Various Stressors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan E Hayes,Lauren M Hemsworth,Rebecca S Morrison,Alan J Tilbrook,Paul H HemsworthPubMed 34070802
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 6.25 - 400 ng/mL
- Assay time
- 40m
- Sensitivity
- = 3.107 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Alternative names
Haptoglobin, HP
What's included?
Recommended products
Pig Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Pig Haptoglobin in Pig in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 6.25 - 400 ng/mL
- Assay time
- 40m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.107 ng/mL
- General recovery
- >= 85%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % >= 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Pig Haptoglobin (ab205091) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological fluid of pigs.
In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
- Biological function summary
Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.
- Pathways
The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.
- Associated diseases and disorders
Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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Sandwich ELISA - Pig Haptoglobin ELISA Kit (ab205091)
Representative standard curve using ab205091 Pig Haptoglobin ELISA Kit.
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