Pig IgA ELISA Kit
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(4 Publications)
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Sandwich ELISA - Pig IgA ELISA Kit (AB190536)
Representative standard curve using ab190536 IgG Pig ELISA Kit.
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Product details
Abcam's IgA Pig ELISA kit (ab190536) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgA in pig serum and plasma.
In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Biological function summary
IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.
Pathways
IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.
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Publications (4)
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Animals : an open access journal from MDPI 11: PubMed34438677
2021
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Food science & nutrition 9:1112-1120 PubMed33598195
2021
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Frontiers in immunology 11:1924 PubMed33013844
2020
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Journal of the science of food and agriculture 99:3616-3627 PubMed30628086
2019
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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