JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190537

Pig IgM ELISA Kit

5

(1 Review)

|

(1 Publication)

Pig IgM ELISA Kit is a Sandwich ELISA for the measurement of Pig IgM in Pig in Biofluids samples.
1 Images
ELISA - Pig IgM ELISA Kit (AB190537)
  • ELISA

Supplier Data

ELISA - Pig IgM ELISA Kit (AB190537)

Representative standard curve using ab190537 IgM Pig ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.072 ng/mL

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB196263

Human IgA ELISA Kit

Recombinant|SimpleStep

ELISA - Human IgA ELISA Kit (AB196263)

  • 4
  • 1
Assay Kits

AB195215

Human IgG ELISA Kit

SimpleStep

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

  • 4
  • 6
Assay Kits

AB189577

Chicken IgY ELISA Kit

SimpleStep

ELISA - Chicken IgY ELISA Kit (AB189577)

  • 0
  • 0
Assay Kits

AB157694

Chicken Ovotransferrin ELISA Kit

Sandwich ELISA - Chicken Ovotransferrin ELISA Kit (AB157694)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IgM Pig ELISA kit (ab190537) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in pig serum and plasma.

In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190537-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB190537-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190537-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IgM Pig HRP Conjugate", "number":"AB190537-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IgM Pig Calibrator (Lyophilized)", "number":"AB190537-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IgM Pig Antibody coated microwells", "number":"AB190537-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190537-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190537-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 11: PubMed34438677

2021

Effects of Coated Cysteamine on Oxidative Stress and Inflammation in Weaned Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Wang,Miaomiao Bai,Kang Xu,Yirui Shao,Zhe Yang,Xia Xiong,Ruilin Huang,Yao Li,Hongnan Liu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com