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AB100754

Pig IL-1 beta ELISA Kit

4

(3 Reviews)

|

(7 Publications)

Pig IL-1 beta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Pig IL-1 beta in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, IL1B

1 Images
Sandwich ELISA - Pig IL-1 beta ELISA Kit (AB100754)
  • sELISA

Supplier Data

Sandwich ELISA - Pig IL-1 beta ELISA Kit (AB100754)

Representative Standard Curve using ab100754

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 104 %", "average": "= 96.91" }, { "sample": "Serum", "range": "88 - 105 %", "average": "= 97.32" }, { "sample": "Plasma", "range": "80 - 92 %", "average": "= 87.75" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100754-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100754-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100754-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL1 beta Microplate (12 x 8 wells)", "number":"AB100754-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Pig IL-1 beta", "number":"AB100754-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100754-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100754-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100754-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Pig IL-1 beta Standard (lyophilized)", "number":"AB100754-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100754-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information IL1B
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:667 PubMed37752585

2023

Tumor microenvironment in a minipig model of spinal cord glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Muhibullah S Tora,Stewart G Neill,Yuliya Lakhina,Hemza Assed,Michelle Zhang,Purva P Nagarajan,Thais Federici,Juanmarco Gutierrez,Kimberly B Hoang,Yuhong Du,Kecheng Lei,Nicholas M Boulis

Cell communication and signaling : CCS 18:23 PubMed32046726

2020

Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhang,Chunxiao Mou,Yanan Cao,En Zhang,Qian Yang

Marine drugs 17: PubMed31835381

2019

Epinecidin-1 Protects against Methicillin Resistant Infection and Sepsis in Pyemia Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ning Huang,Chieh-Yu Pan,Bor-Chyuan Su,Hung-Yi Wu,Jyh-Yih Chen

Journal of applied microbiology 128:355-365 PubMed31618501

2019

Effects of dietary calcium pyruvate on gastrointestinal tract development, intestinal health and growth performance of newly weaned piglets fed low-protein diets.

Applications

Unspecified application

Species

Unspecified reactive species

K Wan,Y Li,W Sun,R An,Z Tang,L Wu,H Chen,Z Sun

Biology of reproduction 102:475-488 PubMed31616930

2019

Ablation of conceptus PTGS2 expression does not alter early conceptus development and establishment of pregnancy in the pig†.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline A Pfeiffer,Ashley E Meyer,Kelsey E Brooks,Paula R Chen,Jessica Milano-Foster,Lee D Spate,Joshua A Benne,Raissa F Cecil,Melissa S Samuel,Lauren A Ciernia,Christine M Spinka,Michael F Smith,Kevin D Wells,Thomas E Spencer,Randall S Prather,Rodney D Geisert

Biology of reproduction 101:148-161 PubMed31066888

2019

New perspective on conceptus estrogens in maternal recognition and pregnancy establishment in the pig†.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley E Meyer,Caroline A Pfeiffer,Kelsey E Brooks,Lee D Spate,Joshua A Benne,Raissa Cecil,Melissa S Samuel,Clifton N Murphy,Susanta Behura,Megan K McLean,Lauren A Ciernia,Michael F Smith,Kristin M Whitworth,Kevin D Wells,Thomas E Spencer,Randall S Prather,Rodney D Geisert

Journal of animal science 96:4723-4730 PubMed30476156

2018

Efficacy of energy supplementation on growth performance and immune response of suckling pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Naiana Einhardt Manzke,Bruna Kuhn Gomes,Eduardo Gonçalves Xavier,Gustavo Julio Mello Monteiro de Lima
View all publications
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