AB207924

Pig Lipocalin-2 ELISA Kit

Name: Pig Lipocalin-2 ELISA Kit (ab207924) | Abcam
Brand: Abcam Limited
SKU: AB207924
Price: 780 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Pig Lipocalin-2 ELISA Kit is a sandwich ELISA designed to quantify Pig Lipocalin-2 with a sensitivity of 1 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 4 - 400 pg/mL

View Alternative Names

LCN2, NGAL

1 Images

Sandwich ELISA - Pig Lipocalin-2 ELISA Kit (AB207924)

sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2 ELISA Kit (AB207924)

Abcam's Pig Lipocalin-2 ELISA Kit (ab207924) Calibration Curve

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

4 - 400 pg/mL

Assay time

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Pig Lipocalin-2 ELISA Kit ab207924 is a sandwich ELISA to measure Pig Lipocalin-2 in serum, plasma, urine with a sensitivity of 1 pg/ml.

How the assay works

The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against pig NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Pig Lipocalin-2 ELISA Kit ab207924 protocol summary

1. Add standards, samples and any controls to appropriate wells
2. Wash
Add Biotinylated monoclonal detection antibody and incubate
4. Wash.
5. Add HRP-conjugated streptavidin.
5. Wash
6. Add TMB
7. Add Stop Solution and read at 450 nm

NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL's ability to bind bacterial siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "P2", "replicates": 8, "mean": "206 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 10" }, { "reproducibilityType": "Inter", "sample": "P1", "replicates": 8, "mean": "134 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 3" }, { "reproducibilityType": "Intra", "sample": "U1", "replicates": 8, "mean": "220 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Inter", "sample": "U1", "replicates": 8, "mean": "220 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Intra", "sample": "U2", "replicates": 8, "mean": "34.2 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Intra", "sample": "P1", "replicates": 8, "mean": "134 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Intra", "sample": "P2", "replicates": 8, "mean": "206 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Inter", "sample": "U2", "replicates": 8, "mean": "34.2 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 5" } ]

style

left-column

Recovery

[ { "sample": "Urine", "range": "96 - 103 %", "average": "= 99" }, { "sample": "Plasma", "range": "96 - 100 %", "average": "= 98" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab207924-1x96Tests", "assetComponentDetails": [ { "size":"1 x 96 Test", "name":"Microwell plate, 96 precoated wells", "number":"AB207924-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (100 pg/mL)", "number":"AB207924-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Biotinylated Pig-NGAL Antibody", "number":"AB207924-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB207924-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-Streptavidin Solution", "number":"AB207924-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (40 pg/mL)", "number":"AB207924-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (400 pg/mL)", "number":"AB207924-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (10 pg/mL)", "number":"AB207924-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"5x Sample Diluent Buffer", "number":"AB207924-CMP02", "productcode":"" }, { "size":"1 x 40 mL", "name":"25x Wash Buffer Solution", "number":"AB207924-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB207924-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (0 pg/mL)", "number":"AB207924-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (20 pg/mL)", "number":"AB207924-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Pig NGAL Standard (200 pg/mL)", "number":"AB207924-CMP11", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

See full target information Lipocalin-2

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Transplant international : official journal of the European Society for Organ Transplantation 38:14268 PubMed40242325

2025

Contrast-Enhanced Ultrasound to Assess Kidney Quality During Normothermic Machine Perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel J Tingle,Chloe Connelly,Emily K Glover,Ben Stenberg,Andrew McNeill,Georgios Kourounis,Beth G Gibson,Balaji Mahendran,Lucy Bates,Madison N Cooper,Rhys R Pook,Samantha Lee,Marnie L Brown,Rodrigo Figueiredo,Kevin J Marchbank,Simi Ali,Neil S Sheerin,Colin H Wilson,Emily R Thompson

Basic research in cardiology 119:957-971 PubMed39404904

2024

Oxidative stress initiates hemodynamic change in CKD-induced heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Payel Sen,Jules Hamers,Theresa Sittig,Bachuki Shashikadze,Laura d'Ambrosio,Jan B Stöckl,Susanne Bierschenk,Hengliang Zhang,Chiara d'Alessio,Lotte M Zandbergen,Valerie Pauly,Sebastian Clauss,Eckhard Wolf,Andreas Dendorfer,Thomas Fröhlich,Daphne Merkus

Frontiers in immunology 13:850271 PubMed35720316

2022

Kidney Normothermic Machine Perfusion Can Be Used as a Preservation Technique and a Model of Reperfusion to Deliver Novel Therapies and Assess Inflammation and Immune Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Azita Mellati,Letizia Lo Faro,Richard Dumbill,Pommelien Meertens,Kaithlyn Rozenberg,Sadr Shaheed,Corinna Snashall,Hannah McGivern,Rutger Ploeg,James Hunter

Antioxidants & redox signaling 35:1407-1425 PubMed33587005

2021

The Microcirculatory Response to Endotoxemia and Resuscitation Is a Marker of Regional Renal Perfusion, Renal Metabolic Stress, and Tubular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hernando Gomez,Håkon Haugaa,Daniel Escobar,Ana M Botero,Rachel Pool,Gaspar Del Rio-Pertuz,Carlos L Manrique-Caballero,Lisa Gordon,Alicia Frank,Jean-Louis Teboul,Brian S Zuckerbraun,Michael R Pinsky

International journal of molecular sciences 21: PubMed32605184

2020

Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Traxler,Andreas Spannbauer,Patrick Einzinger,Julia Mester-Tonczar,Dominika Lukovic,Johannes Winkler,Katrin Zlabinger,Alfred Gugerell,LJubica Mandic,Mariann Gyöngyösi,Noemi Pavo

Redox report : communications in free radical research 24:10-16 PubMed30907266

2019

Early onset of renal oxidative stress in small for gestational age newborn pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Hitesh Soni,Taisiya Yakimkova,Anberitha T Matthews,Paul K Amartey,Robert W Read,Randal K Buddington,Adebowale Adebiyi

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Pig Lipocalin-2 ELISA Kit

Sandwich ELISA - Pig Lipocalin-2 ELISA Kit (AB207924)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise