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AB318928

Pig Lipocalin-2/NGAL ELISA Kit

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Pig Lipocalin-2/NGAL ELISA Kit is a single-wash 90-min Simplestep used to quantify Pig Lipocalin-2/NGAL with a sensitivity of 4.144 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

LCN2, NGAL

5 Images
Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in Göttingen minipig serum in 96-well vs. 384-well plates. Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 Göttingen mini pig serum.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Example of pig Lipocalin-2/NGAL standard curve in Sample Diluent NS. Example of pig Lipocalin-2/NGAL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in Göttingen minipig serum and Yorkshire pig serum. Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations. Undiluted samples are as follows : minipig serum 1 : 400 and Yorkshire pig serum 1 : 1,000.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in minipig plasma (citrate), minipig plasma (EDTA), and minipig plasma (heparin). Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100 minipig plasma (citrate), minipig plasma (EDTA), and minipig plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Example of pig Lipocalin-2/NGAL standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of pig Lipocalin-2/NGAL standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.144 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Pig Lipocalin-2/NGAL SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2/NGAL protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Pig Lipocalin-2/NGAL with 4.144 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "108 - 118 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "93 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "107 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "94 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "94 - 96 %", "average": "= 95" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318928-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318928-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07" }, { "size":"20 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05" }, { "size":"10 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01" }, { "size":"10 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318928-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab318928-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB318928-CMP08" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05" }, { "size":"2 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02" }, { "size":"1 x 384 Wells", "name":"384 well CaptSure™ microplates", "number":"AB318928-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318928-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318928-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318928-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Lipocalin-2
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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