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AB303744

Pig Liver-FABP ELISA Kit

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Pig Liver-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Pig Liver-FABP with a sensitivity of 25.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP, FABP1

2 Images
Sandwich ELISA - Pig Liver-FABP ELISA Kit (AB303744)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Liver-FABP ELISA Kit (AB303744)

Interpolated concentrations of pig Liver-FABP in serum

Interpolated concentration of native Liver-FABP was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 8,000 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Liver-FABP ELISA Kit (AB303744)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Liver-FABP ELISA Kit (AB303744)

Example of pig Liver-FABP standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 25.6 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB222517

Anti-liver FABP antibody [EPR20464]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

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Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

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Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

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Assay Kits

AB239422

Rat Lipocalin-2 ELISA Kit

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Sandwich ELISA - Rat Lipocalin-2 ELISA Kit (AB239422)

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

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  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Pig Liver-FABP SimpleStep ELISA® kit (ab303744) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pig Liver-FABP protein in serum. Quantitate Pig Liver-FABP with 25.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Serum", "range": "0 - 88 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303744-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB303744-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303744-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303744-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303744-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303744-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303744-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Liver-FABP Capture Antibody", "number":"AB303744-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Pig Liver-FABP Lyophilized Recombinant Protein", "number":"AB303744-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Liver-FABP Detector Antibody", "number":"AB303744-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303744-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
See full target information pig L-FABP
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com