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AB190538

Pig Plasminogen ELISA Kit

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(1 Publication)

Pig Plasminogen ELISA Kit is a Sandwich ELISA for the measurement of Pig Plasminogen in Pig in Biofluids samples.

View Alternative Names

Plasminogen, PLG

1 Images
Sandwich ELISA - Pig Plasminogen ELISA Kit (AB190538)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Plasminogen ELISA Kit (AB190538)

Representative standard curve using ab190538 Plasminogen Pig Kit ELISA kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.4137 ng/mL

Range

6.25 - 200 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Plasminogen Pig ELISA kit (ab190538) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Plasminogen in pig serum and plasma.

In this assay the Plasminogen present in samples reacts with the anti-Plasminogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-PMG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound PMG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PMG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PMG in the test sample. The quantity of PMG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Plasminogen (PMG) is a glycoprotein produced by the liver. It is the precursor for plasmin, which targets fibrin in the process of dissolution of fibrin blood clots. Plasminogen is present in plasma and most extravascular fluids. The important role of plasminogen in fibrinolytic system makes it an interesting marker for various diseases.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "< 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190538-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190538-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Diluent Concentrate", "number":"AB190538-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190538-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plasminogen Pig Antibody coated microwells", "number":"AB190538-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Plasminogen Pig HRP Conjugate", "number":"AB190538-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190538-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Plasminogen Pig Calibrator (Lyophilized)", "number":"AB190538-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
Biological function summary

Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.

Pathways

Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.

Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.
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Product protocols

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Target data

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).
See full target information PLG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 32:101258 PubMed38779336

2024

siRNA-mediated reduction of a circulating protein in swine using lipid nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo F Cau,Francesca Ferraresso,Monica Seadler,Katherine Badior,Youjie Zhang,Laura M Ketelboeter,Geoffrey G Rodriguez,Taylor Chen,Matteo Ferraresso,Amanda Wietrzny,Madelaine Robertson,Amber Haugen,Pieter R Cullis,Marc de Moya,Mitchell Dyer,Christian J Kastrup
View all publications
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Product promise

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