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AB108890

Pig Prothrombin ELISA Kit

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Pig Prothrombin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Pig Prothrombin in Pig in Biofluids samples.

View Alternative Names

Prothrombin, Coagulation factor II, F2

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.391 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Prothrombin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of prothrombin levels in plasma and serum samples.

A Prothrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prothrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prothrombin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108890-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108890-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108890-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108890-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108890-CMP01", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Pig Prothrombin Antibody", "number":"AB108890-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108890-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Prothrombin Standard", "number":"AB108890-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Prothrombin Microplate (12 x 8 well strips)", "number":"AB108890-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108890-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prothrombin also known as coagulation factor II is an important protein in blood clotting with an approximate mass of 72 kDa. This protein is synthesized in the liver and is expressed mainly in the plasma as an inactive precursor of thrombin. Prothrombin plays a pivotal role in the conversion into active thrombin through factor X activation in the presence of ionized calcium and phospholipid surfaces. Thrombin then further acts to convert fibrinogen into fibrin leading to clot formation. Prothrombin also can interact with various cellular receptors influencing other pathways beyond coagulation.
Biological function summary

Prothrombin regulates hemostasis and is part of the prothrombinase complex along with factor Xa factor Va calcium ions and phospholipids. This complex allows for the precise regulation of thrombin generation on the surface of activated platelets. By controlling the formation of thrombin prothrombin affects not only blood coagulation but also influences cell proliferation inflammation and tissue repair. The careful balance of prothrombin activation is important to prevent excessive bleeding or clot formation.

Pathways

Prothrombin is an essential component of the coagulation cascade especially the intrinsic and extrinsic coagulation pathways. In these pathways it links with proteins such as fibrinogen and factor X contributing to the amplification and perpetuation of thrombin generation. Additionally thrombin produced from prothrombin engages in a feedback loop to further enhance factor V VIII and XI activation. Through these actions prothrombin helps maintain normal coagulation but must be tightly regulated to prevent thrombotic complications.

Prothrombin is implicated in conditions like prothrombin thrombophilia and liver disease. Prothrombin thrombophilia is a genetic disorder that increases the risk for venous thromboembolism due to the presence of the G20210A mutation in the prothrombin gene. Moreover impaired prothrombin synthesis can occur as a result of liver disease leading to a higher bleeding risk. In thrombotic conditions proteins like protein C and antithrombin work to combat excessive prothrombin-generated thrombin underlining the necessity for balance and regulation in hemostatic processes.
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Product protocols

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (By similarity). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (By similarity).
See full target information F2
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Product promise

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