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AB100756

Pig TNF alpha ELISA Kit

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(10 Publications)

Pig TNF alpha ELISA Kit is a sandwich ELISA designed to quantify Pig TNF alpha with a sensitivity of 20 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.049 - 10 ng/mL

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Sandwich ELISA - Pig TNF alpha ELISA Kit (AB100756)
  • sELISA

Supplier Data

Sandwich ELISA - Pig TNF alpha ELISA Kit (AB100756)

Representative Standard Curve using ab100756

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

0.049 - 10 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Pig TNF alpha ELISA Kit ab100756 is a sandwich ELISA to measure Pig TNF alpha in serum, plasma, cell culture supernatant with a sensitivity of 20 pg/ml.

How the assay works

This assay employs an antibody specific for Pig TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Pig TNF alpha ELISA Kit ab100756 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Recovery

[ { "sample": "Cell culture supernatant", "range": "123 - 142 %", "average": "= 132.1" }, { "sample": "Serum", "range": "102 - 121 %", "average": "= 111.7" }, { "sample": "Plasma", "range": "70 - 126 %", "average": "= 97.02" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100756-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100756-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100756-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100756-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Pig TNF alpha Standard (lyophilized)", "number":"AB100756-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100756-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 wells)", "number":"AB100756-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100756-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Pig TNF alpha", "number":"AB100756-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100756-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:667 PubMed37752585

2023

Tumor microenvironment in a minipig model of spinal cord glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Muhibullah S Tora,Stewart G Neill,Yuliya Lakhina,Hemza Assed,Michelle Zhang,Purva P Nagarajan,Thais Federici,Juanmarco Gutierrez,Kimberly B Hoang,Yuhong Du,Kecheng Lei,Nicholas M Boulis

Clinical transplantation 35:e14417 PubMed34231926

2021

Ischemic postconditioning improves the outcome of organs from donors after cardiac death in a pig liver transplantation model and provides synergistic protection with hypothermic machine perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Degong Jia,Qi Pan,Yijie Zhang,Yang Yu,Zhanyu Song,Yong Feng Liu,Zhixing Jia,Shanshan Guo,Ying Cheng

Cell communication and signaling : CCS 18:23 PubMed32046726

2020

Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhang,Chunxiao Mou,Yanan Cao,En Zhang,Qian Yang

Marine drugs 17: PubMed31835381

2019

Epinecidin-1 Protects against Methicillin Resistant Infection and Sepsis in Pyemia Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ning Huang,Chieh-Yu Pan,Bor-Chyuan Su,Hung-Yi Wu,Jyh-Yih Chen

Journal of applied microbiology 128:355-365 PubMed31618501

2019

Effects of dietary calcium pyruvate on gastrointestinal tract development, intestinal health and growth performance of newly weaned piglets fed low-protein diets.

Applications

Unspecified application

Species

Unspecified reactive species

K Wan,Y Li,W Sun,R An,Z Tang,L Wu,H Chen,Z Sun

Cardiovascular diabetology 18:107 PubMed31429767

2019

Dapagliflozin improves left ventricular remodeling and aorta sympathetic tone in a pig model of heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Zhang,Bin Feng,Xuexing Ma,Kangyun Sun,Guidong Xu,Yafeng Zhou

Journal of the science of food and agriculture 99:3616-3627 PubMed30628086

2019

Effects of pulverized oyster mushroom (Pleurotus ostreatus) on diarrhea incidence, growth performance, immunity, and microbial composition in piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Seidu Adams,Dongsheng Che,Jiang Hailong,Bao Zhao,Han Rui,Kofi Danquah,Guixin Qin

Annals of transplantation 24:9-17 PubMed30607000

2019

Normothermic Machine Perfusion Protects Against Liver Ischemia-Reperfusion Injury During Reduced-Size Liver Transplantation in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Bin Zhang,Wei Gao,Lei Liu,Yuan Shi,Ning Ma,Ming-Sheng Huai,Zhong-Yang Shen

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 18:855-867 PubMed29087049

2017

Anti-CD47 monoclonal antibody therapy reduces ischemia-reperfusion injury of renal allografts in a porcine model of donation after cardiac death.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xu,Xuanchuan Wang,Babak Banan,Danielle L Chirumbole,Sandra Garcia-Aroz,Aparna Balakrishnan,Deepak K Nayak,Zhengyan Zhang,Jianluo Jia,Gundumi A Upadhya,Joseph P Gaut,Ronald Hiebsch,Pamela T Manning,Ningying Wu,Yiing Lin,William C Chapman

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 24:1089-1096 PubMed27717144

2016

An ex vivo porcine skin model to evaluate pressure-reducing devices of different mechanical properties used for pressure ulcer prevention.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Yan C Yeung,David F Holmes,Helen A Thomason,Christian Stephenson,Brian Derby,Matthew J Hardman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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