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Polyethylene Glycol ELISA Kit is a Competitive ELISA kit for the measurement of Polyethylene Glycol in Plasma, Cell culture media, Serum samples.


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Competitive ELISA - Polyethylene Glycol ELISA Kit (AB215546), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Assay type

Competitive

Range

2 - 200 ng/mL

Reactivity data

Application

cELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

1 x 96 Tests
Components
10X Wash Buffer PT (ab206977)
1 x 20 mL
Anti-PEG coated microplate (12 x 8 well strips)
1 x 1 Unit
Antigen/Antibody Diluent Buffer 1X
1 x 20 mL
PEG-BSA Reference Standard (Lyophilized)
1 x 200 ng
PEG-conjugated HRP 60X
1 x 150 µL
Plate Seals
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Polyethylene Glycol ELISA Kit is a Competitive ELISA kit for the measurement of Polyethylene Glycol in Plasma, Cell culture media, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Assay type

Competitive

Range

2 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Precision

Intra assay

Sample

Overall

n

4

mean

-

SD

-

C.V.

3

Inter assay

Sample

Overall

n

4

mean

-

SD

-

C.V.

6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 91

Range

82 - 101 %

Sample type

Cell culture media

Average %

= 104

Range

99 - 108 %

Sample type

Citrate plasma

Average %

= 79

Range

75 - 85 %

Sample type

EDTA Plasma

Average %

= 84

Range

77 - 90 %

Sample type

Heparin Plasma

Average %

= 70

Range

64 - 77 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Polyethylene Glycol (PEG) RabMAb®in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media.

The Polyethylene Glycol (PEG) RabMAb® ELISA Kit operates on the basis of competition between enzyme HRP conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG RabMab® antibody. The extent of color development resulting from interaction between HRP and the substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample. For example, the absence of PEGylated molecules in the sample will result in a bright blue color, whereas the presence of PEGylated molecules will result in decreased or no color development.

This kit is developed to determine levels of PEGylated molecules in samples such as serum, plasma or cell culture medium via ELISA.

Users must PEGylate their interested molecules. For pharmaco kinetic experiments, the PEGylated molecules will be used to construct standard curves.

The use of this kit requires the end user to have at least 250 ng of PEGylated compound of interest to use as reference standard. The included PEG-BSA is a reference sample only.

Polyethylene glycol (PEG) is an O-CH2-CH2 polymer, which is water-soluble, nontoxic, non-antigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases the in vivo stability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance. Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers. Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical application.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Competitive ELISA - Polyethylene Glycol ELISA Kit (ab215546), expandable thumbnail

    Competitive ELISA - Polyethylene Glycol ELISA Kit (ab215546)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com