Polyethylene Glycol ELISA Kit is a Competitive ELISA kit for the measurement of Polyethylene Glycol in Plasma, Cell culture media, Serum samples.
Colorimetric
Plasma, Cell culture media, Serum
Competitive
2 - 200 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
PEG
Polyethylene Glycol ELISA Kit is a Competitive ELISA kit for the measurement of Polyethylene Glycol in Plasma, Cell culture media, Serum samples.
Colorimetric
Plasma, Cell culture media, Serum
Competitive
2 - 200 ng/mL
Pre-coated microplate (12 x 8 well strips)
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 4 | mean - | SD - | C.V. 3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 4 | mean - | SD - | C.V. 6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 91 | Range 82 - 101 % |
Sample type Cell culture media | Average % = 104 | Range 99 - 108 % |
Sample type Citrate plasma | Average % = 79 | Range 75 - 85 % |
Sample type EDTA Plasma | Average % = 84 | Range 77 - 90 % |
Sample type Heparin Plasma | Average % = 70 | Range 64 - 77 % |
Blue Ice
+4°C
+4°C
+4°C
Polyethylene Glycol (PEG) RabMAb®in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media.
The Polyethylene Glycol (PEG) RabMAb® ELISA Kit operates on the basis of competition between enzyme HRP conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG RabMab® antibody. The extent of color development resulting from interaction between HRP and the substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample. For example, the absence of PEGylated molecules in the sample will result in a bright blue color, whereas the presence of PEGylated molecules will result in decreased or no color development.
This kit is developed to determine levels of PEGylated molecules in samples such as serum, plasma or cell culture medium via ELISA.
Users must PEGylate their interested molecules. For pharmaco kinetic experiments, the PEGylated molecules will be used to construct standard curves.
The use of this kit requires the end user to have at least 250 ng of PEGylated compound of interest to use as reference standard. The included PEG-BSA is a reference sample only.
Polyethylene glycol (PEG) is an O-CH2-CH2 polymer, which is water-soluble, nontoxic, non-antigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases the in vivo stability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance. Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers. Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical application.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com