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AB273215

Porcine CCL4 ELISA Kit

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Porcine CCL4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine CCL4 in Pig in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.
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Sandwich ELISA - Porcine CCL4 ELISA Kit (AB273215)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine CCL4 ELISA Kit (AB273215)

Porcine CCL4 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Cell culture supernatant

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine CCL4 ELISA Kit (ab273215) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine CCL4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Porcine CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Porcine CCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Porcine CCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Plasma", "range": "93 - 121 %", "average": "= 104.8" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 122.1" }, { "sample": "Serum", "range": "106 - 118 %", "average": "= 111.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273215-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273215-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273215-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine CCL4 coated Microplate (12 x 8 wells)", "number":"AB273215-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273215-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine CCL4 Standard (Lyophilized)", "number":"AB273215-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine CCL4 Antibody", "number":"AB273215-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB273215-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273215-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
Biological function summary

CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.

Pathways

CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.

CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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Product protocols

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Target data

See full target information CCL4
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websiteProtocolBooklet
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