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Porcine CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine CXCL9 in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
Sandwich (quantitative)
- Range
5 - 1400 pg/mL
- Sensitivity
= 5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Porcine CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine CXCL9 in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
Sandwich (quantitative)
- Range
5 - 1400 pg/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88.25 | Range 68 - 116 % |
Sample type Cell culture media | Average % = 94.1 | Range 70 - 123 % |
Sample type Plasma | Average % = 105.4 | Range 78 - 139 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Porcine CXCL9 ELISA Kit (ab273210) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine CXCL9 in serum, plasma (collect plasma using EDTA or citrate as an anti-coagulant. Heparin is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Product protocols
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