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AB273221

Porcine Decorin ELISA Kit

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Porcine Decorin ELISA Kit is a Sandwich ELISA for the measurement of Porcine Decorin in Human, Pig in Cell Culture Media, Biofluids samples.

View Alternative Names

Decorin, Bone proteoglycan II, PG-S2, DCN

1 Images
Sandwich ELISA - Porcine Decorin ELISA Kit (AB273221)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine Decorin ELISA Kit (AB273221)

Porcine Decorin Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Porcine Decorin ELISA kit (ab273221) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Human, Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine Decorin ELISA Kit 9ab273221)) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Decorin in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for porcine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 104 %", "average": "= 97.43" }, { "sample": "Serum", "range": "92 - 142 %", "average": "= 116.8" }, { "sample": "Plasma", "range": "67 - 91 %", "average": "= 93.49" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273221-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273221-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine Decorin Standard (Lyophilized)", "number":"AB273221-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273221-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273221-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273221-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine Decorin coated Microplate (12 x 8 wells)", "number":"AB273221-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine Decorin Antibody", "number":"AB273221-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273221-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May affect the rate of fibrils formation.
See full target information DCN
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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