Porcine Decorin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine Decorin in Human, Pig in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Pig
- Range
- 4.1 - 1000 pg/mL
- Sensitivity
- = 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Decorin, Bone proteoglycan II, PG-S2, DCN
What's included?
Recommended products
Porcine Decorin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine Decorin in Human, Pig in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Pig
- Range
- 4.1 - 1000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97.43 | Range 93 - 104 % |
Sample type Serum | Average % = 116.8 | Range 92 - 142 % |
Sample type Plasma | Average % = 93.49 | Range 67 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Porcine Decorin ELISA Kit 9ab273221)) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Decorin in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Terms & Conditions.
1 product image
Sandwich ELISA - Porcine Decorin ELISA Kit (ab273221)
Porcine Decorin Standard Curve.
Typical standard curve– data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine Decorin ELISA kit (ab273221) Standard curve.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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