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AB273220

Porcine FGF19 ELISA Kit

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(1 Publication)

Porcine FGF19 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine FGF19 in Pig in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Porcine FGF19 ELISA Kit (AB273220)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine FGF19 ELISA Kit (AB273220)

Porcine FGF-19 Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Porcine FGF-19 ELISA kit (ab273220) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 10 pg/mL

Range

20.48 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine FGF19 ELISA Kit (ab273220) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine FGF-19 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for porcine FGF-19 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF-19 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine FGF-19 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-19 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 145 %", "average": "= 112.9" }, { "sample": "Serum", "range": "135 - 149 %", "average": "= 141.8" }, { "sample": "Plasma", "range": "127 - 146 %", "average": "= 139.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273220-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273220-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine FGF19 coated Microplate (12 x 8 wells)", "number":"AB273220-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine FGF19 Antibody", "number":"AB273220-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB273220-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273220-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine FGF19 Standard (Lyophilized)", "number":"AB273220-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273220-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273220-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF19 also known as fibroblast growth factor 19 is a 251 amino acid protein with a molecular mass of approximately 27 kDa. It is mostly expressed in the liver small intestine and to some extent in the gallbladder. FGF19 is related to the FGF family of growth factors which are involved in a variety of developmental and physiological processes. Unlike many other FGF members FGF19 lacks a heparin-binding domain which influences its receptor binding characteristics. Researchers frequently use FGF19 ELISA kits and FGF19 assays to study this protein due to its significance in regulating bile acid metabolism.
Biological function summary

Fibroblast growth factor 19 participates in maintaining metabolic homeostasis and controlling bile acid synthesis. It acts by binding to a receptor complex composed of FGFR4 and beta-Klotho which signals the regulation of energy balance and interaction with other metabolic targets. FGF19 protein triggers pathways that modulate glucose and lipid metabolism making it an important factor in metabolic health. Its regulation of lipid and glucose processes emphasizes its role in metabolic control systems and potential impacts on obesity and diabetes management.

Pathways

The fibroblast growth factor 19 engages in two critical signaling routes: the MAPK/ERK and the PI3K/AKT pathways. These pathways are fundamental in cell proliferation differentiation and survival. FGF19 through FGFR4 activation influences regulatory proteins and factors downstream bridging interactions with other key molecules such as insulin and leptin pathways. This regulatory mechanism contributes to its ability to fine-tune a wide range of metabolic functions and influences energy balance.

Fibroblast growth factor 19 shows a significant connection to non-alcoholic fatty liver disease (NAFLD) and certain forms of cancer. Altered expression levels of FGF19 associate with liver conditions due to its role in bile acid regulation and energy homeostasis. In cancer particularly hepatocellular carcinoma the dysregulation of FGF19 and its receptor plays a part in unchecked cellular proliferation and tumorigenesis. Researchers have noted connections with proteins such as FGFR4 and beta-Klotho highlighting their collaborative roles in disease progression and potential therapeutic targeting strategies.
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Product protocols

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Target data

See full target information FGF19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 600:1771-1790 PubMed35081669

2022

KLB dysregulation mediates disrupted muscle development in intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Yennifer Cortes-Araya,Claire Stenhouse,Mazdak Salavati,Susan O Dan-Jumbo,William Ho,Cheryl J Ashworth,Emily Clark,Cristina L Esteves,F Xavier Donadeu
View all publications
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