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AB273207

Porcine IL-12 ELISA Kit

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Porcine IL-12 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine IL-12 in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, IL12B

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ELISA - Porcine IL-12 ELISA Kit (AB273207)
  • ELISA

Supplier Data

ELISA - Porcine IL-12 ELISA Kit (AB273207)

Porcine IL-12 ELISA Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Porcine IL-12 ELISA kit (ab273207) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 2.2 ng/mL

Range

2.458 - 600 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine IL-12 ELISA Kit (ab273207) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-12 in serum (Porcine IL-12 concentration is low in normal serum/plasma and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Porcine IL-12 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- Porcine IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 81 %", "average": "= 78.43" }, { "sample": "Plasma", "range": "68 - 83 %", "average": "= 72.71" }, { "sample": "Cell culture media", "range": "68 - 130 %", "average": "= 98.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273207-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273207-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273207-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine IL-12 Antibody", "number":"AB273207-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273207-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine IL-12 Standard (Lyophilized)", "number":"AB273207-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine IL-12 coated Microplate (12 x 8 wells)", "number":"AB273207-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273207-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"1800X HRP-Streptavidin Concentrate", "number":"AB273207-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273207-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis (By similarity).
See full target information IL12B
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websiteProtocolBooklet
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