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AB273224

Porcine IL-17F ELISA Kit

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Porcine IL-17F ELISA Kit is a Sandwich ELISA for the measurement of Porcine IL-17F in Pig, Rat in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Porcine IL-17F ELISA Kit (AB273224)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine IL-17F ELISA Kit (AB273224)

Porcine IL-17F Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Porcine IL-17F ELISA kit (ab273224) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Rat, Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine IL-17F ELISA Kit (ab273224) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-17F in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulant for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Porcine IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "73 - 84 %", "average": "= 76.04" }, { "sample": "Serum", "range": "63 - 71 %", "average": "= 74.84" }, { "sample": "Plasma", "range": "75 - 91 %", "average": "= 82.75" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273224-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273224-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine IL-17F coated Microplate (12 x 8 wells)", "number":"AB273224-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273224-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine IL-17F Standard (Lyophilized)", "number":"AB273224-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273224-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273224-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273224-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB273224-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine IL-17F Antibody", "number":"AB273224-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information IL-17F
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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