Porcine IL-17F ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine IL-17F in Rat, Pig in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat, Pig
- Range
- 4.1 - 1000 pg/mL
- Sensitivity
- = 6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
Porcine IL-17F ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine IL-17F in Rat, Pig in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat, Pig
- Range
- 4.1 - 1000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 76.04 | Range 73 - 84 % |
Sample type Serum | Average % = 74.84 | Range 63 - 71 % |
Sample type Plasma | Average % = 82.75 | Range 75 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Porcine IL-17F ELISA Kit (ab273224) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-17F in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulant for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Porcine IL-17F ELISA Kit (ab273224)
Porcine IL-17F Standard Curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine IL-17F ELISA kit (ab273224) Standard curve.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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