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AB273212

Porcine IL-22 ELISA Kit

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Porcine IL-22 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine IL-22 in Pig in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Porcine IL-22 ELISA Kit (AB273212)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine IL-22 ELISA Kit (AB273212)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 20 pg/mL

Range

20 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine IL-22 ELISA Kit (ab273212) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-22 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for porcine IL-22 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-22 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine IL-22 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-22 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 97 %", "average": "= 91.41" }, { "sample": "Plasma", "range": "68 - 81 %", "average": "= 70.97" }, { "sample": "Cell culture extracts", "range": "88 - 110 %", "average": "= 98.98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273212-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273212-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273212-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273212-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273212-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine IL-22 Antibody", "number":"AB273212-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273212-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine IL-22 Standard (Lyophilized)", "number":"AB273212-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine IL-22 coated Microplate (12 x 8 wells)", "number":"AB273212-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information IL22
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websiteProtocolBooklet
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Product promise

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