Porcine IL-22 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine IL-22 in Pig in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 20 - 6000 pg/mL
- Sensitivity
- = 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
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Porcine IL-22 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine IL-22 in Pig in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 20 - 6000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 20 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91.41 | Range 83 - 97 % |
Sample type Plasma | Average % = 70.97 | Range 68 - 81 % |
Sample type Cell culture extracts | Average % = 98.98 | Range 88 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Porcine IL-22 ELISA Kit (ab273212) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-22 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine IL-22 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-22 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine IL-22 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-22 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Porcine IL-22 ELISA Kit (ab273212)
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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