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AB273205

Porcine IL-4 ELISA Kit

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Porcine IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine IL-4 in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

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ELISA - Porcine IL-4 ELISA Kit (AB273205)
  • ELISA

Supplier Data

ELISA - Porcine IL-4 ELISA Kit (AB273205)

Porcine IL-4 ELISA Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Porcine IL-4 ELISA kit (ab273205) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5 pg/mL

Range

1.638 - 400 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine IL-4 ELISA Kit (ab273205) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-4 in serum (Porcine IL-4 concentration is low in normal serum/plasma and may not be detectable in this assay), plasma, and cell culture supernatants.

This assay employs an antibody specific for porcine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti porcine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "71 - 134 %", "average": "= 101" }, { "sample": "Plasma", "range": "72 - 126 %", "average": "= 89.54" }, { "sample": "Cell culture media", "range": "68 - 133 %", "average": "= 99.28" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273205-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273205-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273205-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine IL-4 coated Microplate (12 x 8 wells)", "number":"AB273205-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine IL-4 Standard (Lyophilized)", "number":"AB273205-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273205-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273205-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine IL-4 Antibody", "number":"AB273205-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273205-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4
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