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AB273214

Porcine MCP1 ELISA Kit

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Porcine MCP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine MCP1 in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

SCYA2, CCL2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

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Sandwich ELISA - Porcine MCP1 ELISA Kit (AB273214)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine MCP1 ELISA Kit (AB273214)

Porcine MCP1 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 28 pg/mL

Range

28.67 - 7000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine MCP1 ELISA Kit (ab273214) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine MCP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Porcine MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Porcine MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Porcine MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 149 %", "average": "= 111.2" }, { "sample": "Plasma", "range": "68 - 132 %", "average": "= 100.4" }, { "sample": "Cell culture media", "range": "69 - 100 %", "average": "= 81.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273214-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273214-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine MCP1 Standard (Lyophilized)", "number":"AB273214-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"1000X HRP-Streptavidin Concentrate", "number":"AB273214-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273214-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine MCP1 Antibody", "number":"AB273214-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273214-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273214-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine MCP1 coated Microplate (12 x 8 wells)", "number":"AB273214-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
See full target information CCL2
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