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AB273217

Porcine Osteoprotegerin ELISA Kit (OPG)

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Porcine Osteoprotegerin ELISA Kit (OPG) is a Sandwich ELISA for the measurement of Porcine Osteoprotegerin (OPG) in Pig in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Porcine Osteoprotegerin ELISA Kit (OPG) (AB273217)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine Osteoprotegerin ELISA Kit (OPG) (AB273217)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.12 ng/mL

Range

0.123 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB73400

Anti-Osteoprotegerin antibody

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Human TNFSF11 ELISA Kit (RANKL)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB192256

Anti-RUNX2 antibody [EPR14334]

Recombinant|Advanced Validation|RabMAb|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] (AB192256)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine Osteoprotegerin ELISA Kit (OPG) (ab273217) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Osteoprotegerin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for porcine Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-porcine Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 89 %", "average": "= 85.09" }, { "sample": "Plasma", "range": "90 - 120 %", "average": "= 103.3" }, { "sample": "Cell culture media", "range": "77 - 104 %", "average": "= 89.47" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273217-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273217-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin Concentrate", "number":"AB273217-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273217-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273217-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273217-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine Osteoprotegerin (OPG) coated Microplate (12 x 8 wells)", "number":"AB273217-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine Osteoprotegerin (OPG) Antibody", "number":"AB273217-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine Osteoprotegerin (OPG) Standard (Lyophilized)", "number":"AB273217-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
Biological function summary

OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.

Pathways

OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.

OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
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Product protocols

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Target data

See full target information Osteoprotegerin
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websiteProtocolBooklet
en
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Product promise

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