JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273218

Porcine RANTES ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Porcine RANTES ELISA Kit is a Sandwich ELISA for the measurement of Porcine RANTES in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

SCYA5, CCL5, C-C motif chemokine 5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES

1 Images
Sandwich ELISA - Porcine RANTES ELISA Kit (AB273218)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine RANTES ELISA Kit (AB273218)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 46.9 pg/mL

Range

46.88 - 3000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB173194

Human IP-10 ELISA Kit

SimpleStep

ELISA - Human IP-10 ELISA Kit (AB173194)

  • 5
  • 1
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB179886

Human MCP-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MCP-1 ELISA Kit (AB179886)

  • 0
  • 0
Assay Kits

AB214030

Human IL-8 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Porcine RANTES ELISA Kit (ab273218) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine RANTES in serum (porcine RANTES concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for porcine RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-porcine RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "86 - 90 %", "average": "= 88.59" }, { "sample": "Plasma", "range": "67 - 91 %", "average": "= 75.86" }, { "sample": "Cell culture media", "range": "93 - 104 %", "average": "= 97.43" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273218-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273218-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273218-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273218-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine RANTES coated Microplate (12 x 8 wells)", "number":"AB273218-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine RANTES Antibody", "number":"AB273218-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine RANTES Standard (Lyophilized)", "number":"AB273218-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273218-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273218-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
See full target information CCL5
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com