Porcine RANTES ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine RANTES in Pig in Plasma, Cell culture supernatant, Serum samples.
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
SCYA5, CCL5, C-C motif chemokine 5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES
Porcine RANTES ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine RANTES in Pig in Plasma, Cell culture supernatant, Serum samples.
Sample | n | C.V. |
---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Sample | n | C.V. |
---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 88.59 | Range 86 - 90 % |
Sample type Plasma | Average % = 75.86 | Range 67 - 91 % |
Sample type Cell culture media | Average % = 97.43 | Range 93 - 104 % |
Porcine RANTES ELISA Kit (ab273218) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine RANTES in serum (porcine RANTES concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for porcine RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-porcine RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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