Porcine TGF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine TGF alpha in Pig in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 0.49 - 120 pg/mL
- Sensitivity
- = 0.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Alternative names
Protransforming growth factor alpha, TGFA
What's included?
Recommended products
Porcine TGF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine TGF alpha in Pig in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 0.49 - 120 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 128.6 | Range 126 - 133 % |
Sample type Plasma | Average % = 122.5 | Range 88 - 142 % |
Sample type Cell culture extracts | Average % = 129.9 | Range 114 - 142 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Porcine TGF-alpha ELISA Kit (ab273225) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine TGF-alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine TGF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine TGF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product promise
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1 product image
Sandwich ELISA - Porcine TGF alpha ELISA Kit (ab273225)
Porcine TGF alpha ELISA Kit standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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