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AB273225

Porcine TGF alpha ELISA Kit

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Porcine TGF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine TGF alpha in Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

Protransforming growth factor alpha, TGFA

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Sandwich ELISA - Porcine TGF alpha ELISA Kit (AB273225)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine TGF alpha ELISA Kit (AB273225)

Porcine TGF alpha ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 0.6 pg/mL

Range

0.49 - 120 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine TGF-alpha ELISA Kit (ab273225) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine TGF-alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Porcine TGF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine TGF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "126 - 133 %", "average": "= 128.6" }, { "sample": "Plasma", "range": "88 - 142 %", "average": "= 122.5" }, { "sample": "Cell culture extracts", "range": "114 - 142 %", "average": "= 129.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273225-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273225-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine TGF alpha Antibody", "number":"AB273225-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine TGF alpha Standard (Lyophilized)", "number":"AB273225-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine TGF alpha coated Microplate (12 x 8 wells)", "number":"AB273225-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273225-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273225-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273225-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB273225-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
See full target information TGFA
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websiteProtocolBooklet
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Product promise

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