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AB273209

Porcine TIMP2 ELISA Kit

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Porcine TIMP2 ELISA Kit is a Sandwich ELISA for the measurement of Porcine TIMP2 in in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Porcine TIMP2 ELISA Kit (AB273209)
  • sELISA

Supplier Data

Sandwich ELISA - Porcine TIMP2 ELISA Kit (AB273209)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 28 pg/mL

Range

28 - 7000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

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Assay Kits

AB187394

Human TIMP1 ELISA Kit

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Porcine TIMP2 ELISA Kit (ab273209) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine TIMP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for porcine TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "71 - 112 %", "average": "= 89.62" }, { "sample": "Plasma", "range": "68 - 110 %", "average": "= 87.79" }, { "sample": "Cell culture media", "range": "68 - 98 %", "average": "= 82.13" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273209-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273209-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Porcine TIMP2 coated Microplate (12 x 8 wells)", "number":"AB273209-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273209-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Porcine TIMP2 Standard (Lyophilized)", "number":"AB273209-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273209-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Porcine TIMP2 Antibody", "number":"AB273209-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273209-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273209-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

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Target data

See full target information TIMP2
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websiteProtocolBooklet
en
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Product promise

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