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Porcine TIMP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine TIMP2 in Plasma, Cell culture supernatant, Serum samples.
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Range
28 - 7000 pg/mL
- Sensitivity
= 28 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Porcine TIMP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Porcine TIMP2 in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Range
28 - 7000 pg/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 28 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89.62 | Range 71 - 112 % |
Sample type Plasma | Average % = 87.79 | Range 68 - 110 % |
Sample type Cell culture media | Average % = 82.13 | Range 68 - 98 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Porcine TIMP2 ELISA Kit (ab273209) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine TIMP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product promise
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Full details and terms and conditions can be found here:
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Product protocols
- Visit the general protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com