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AB133024

Prostaglandin E1 ELISA Kit

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Prostaglandin E1 ELISA Kit is a Competitive ELISA for the measurement of Prostaglandin E1 in Human in Biofluids, Cell Culture Media samples.
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Competitive ELISA - Prostaglandin E1 ELISA Kit (AB133024)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E1 ELISA Kit (AB133024)

Representative Standard Curve using ab133024

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Serum, Tissue Culture Media

Reacts with

Human

Assay type

Competitive

Sensitivity

= 5.58 pg/mL

Range

4.88 - 5000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Prostaglandin E1 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E1 in, plasma, serum, urine, tissue culture media and other biological fluids.

A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE1 antigen and a polyclonal sheep antibody specific to PGE1. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the Alkaline Phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE1 captured in the plate.

Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.

Cross Reactivity

Compound Cross Reactivity
PGE1 100%
PGE2 6.50%
PGE3 2.22%
13,14-dihydro-PGE1 1.50%
PGE0 1.45%
15-keto-PGE1 1.15%
13,14-dihydro-15-keto-PGE1 0.19%
PGF1α 0.14%
PGF2α 0.04%
6-keto-PGF1α <0.1%
PGA2 <0.1%
PGD2 <0.1%
PGB1 <0.1%
13,14-dihydro-15-keto-PGF2α <0.1%
6,15-keto-13,14-dihydro-PGF1α <0.1%
Thromboxane B2 <0.1%
Misoprostol <0.1%
2-Arachidonoylglycerol <0.1%
Anandamide <0.1%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "737 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 8, "mean": "49 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 8, "mean": "214 pg/mL", "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 24, "mean": "1103 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13.7" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 24, "mean": "53 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 24, "mean": "246 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.5" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 87" }, { "sample": "Plasma", "range": null, "average": "= 107.7" }, { "sample": "Urine", "range": null, "average": "= 109.9" }, { "sample": "Saliva", "range": null, "average": "= 107.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133024-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133024-CMP04", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133024-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"PGE1 Antibody", "number":"AB133024-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Donkey anti-sheep IgG Microplate (12 x 8 wells)", "number":"AB133024-CMP08", "productcode":"" }, { "size":"1 x 500 µL", "name":"PGE1 Standard", "number":"AB133024-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"PGE1 Alkaline Phosphatase Conjugate", "number":"AB133024-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133024-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133024-CMP07", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133024-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E1 (PGE1) also known as alprostadil is a lipid compound with a mass of around 354.49 g/mol. It functions as a vasodilator and inhibitor of platelet aggregation. PGE1 achieves its effects by increasing the levels of cAMP through the activation of adenylate cyclase. This natural compound is expressed in tissues like the kidneys lungs and seminal vesicles reflecting its diverse roles in the body. Its action results in the relaxation of smooth muscle tissues which is important for its role in various physiological processes.
Biological function summary

PGE1 participations involve modulation of inflammation and immune response. It is part of the prostaglandin family playing significant roles in processes like vasodilation and bronchodilation. Also PGE1 is known to modulate cellular second messengers affecting various biological functions such as cell growth and differentiation. As a bioactive lipid PGE1 interacts with other components such as ginkgo biloba extracts and various intracellular proteins to enhance its biological activity.

Pathways

PGE1 participates in signaling networks like the COX (cyclooxygenase) pathway and the cAMP-mediated signaling pathway. These pathways help regulate processes such as inflammation and platelet function. In the COX pathway PGE1 synthesis involves the conversion of arachidonic acid an important reaction regulated by cyclooxygenase enzymes. In these pathways PGE1 often interacts with proteins such as EP receptors which mediate its cellular effects linking it to related pathways and their biological responses.

PGE1 has relations to conditions such as peripheral vascular disease and erectile dysfunction. PGE1’s vasodilatory effect helps improve blood flow which is beneficial in treating these conditions. For instance in peripheral vascular disease PGE1 helps reduce symptoms by enhancing blood circulation. Its interaction with proteins like the nitric oxide synthase further potentiates its ability to address vascular issues offering therapeutic implications to manage these disorders effectively.
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Product protocols

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Target data

See full target information PGE1
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websiteProtocolBooklet
en
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