JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316263

Prostaglandin E2 ELISA Kit - Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Prostaglandin E2 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Prostaglandin E2 with a sensitivity of 4.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

PGE2, Dinoprostone, Prostin E2

6 Images
Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Serum from nine individual healthy female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Prostaglandin E2 concentration was determined to be 234 pg/mL with a range of 92 – 585 pg/mL.

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Interpolated concentration of native Prostaglandin E2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% Human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent ES.

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

​​Prostaglandin E2​ was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent ES. Undiluted samples are 50% Human urine, and cell culture media with or without 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Example of  Prostaglandin E2 standard curve.  OD (450 nm) values (mean +/- SD) are graphed.

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Prostaglandin E2 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent ES. Undiluted samples are 50% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Serum from ten individual healthy male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Prostaglandin E2 concentration was determined to be 335 pg/mL with a range of 67 – 837 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Urine, Citrate plasma, Heparin Plasma, Cell culture media

Assay type

Competitive

Sensitivity

= 4.75 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Prostaglandin E2 SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Prostaglandin E2 in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, and Cell culture media. Quantitate Prostaglandin E2 with 4.75 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 13.9" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "83 - 117 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 110 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "88 - 101 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "Urine", "range": "88 - 104 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "107 - 109 %", "average": "= 108" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab316263-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB316263-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316263-CMP06" }, { "size":"1 x 200 mL", "name":"Sample Diluent ES", "number":"AB316263-CMP07" }, { "size":"10 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316263-CMP10" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB316263-CMP01" }, { "size":"10 x 2 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316263-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB316263-CMP03" }, { "size":"10 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316263-CMP08" }, { "size":"10 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316263-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316263-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316263-96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316263-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent ES", "number":"AB316263-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316263-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316263-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316263-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316263-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316263-CMP08", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316263-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316263-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316263-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316263-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E2 (PGE2) also known as dinoprostone is a lipid compound with a molecular weight of approximately 352.47 g/mol. This compound is synthesized in various tissues like the kidneys lungs and brain primarily by the enzymatic action of cyclooxygenase (COX) on arachidonic acid. As a member of the prostaglandin family PGE2 functions as an important signaling molecule and mediator of inflammation. Researchers often use PGE2 ELISA kits and assays including those specific for mouse PGE2 to measure its levels accurately in biological samples.
Biological function summary

As a potent mediator PGE2 plays roles in inflammation fever induction and regulation of vascular tone. It exerts its effects through specific G-protein-coupled receptors (EP1 EP2 EP3 and EP4) on the surface of target cells. Although not part of a strict protein complex PGE2 works in conjunction with other inflammatory mediators including cytokines and other prostaglandins. It influences cellular activities by modulating the intracellular cyclic AMP levels and calcium fluxes.

Pathways

PGE2 is an important component of the prostaglandin biosynthesis pathway and the broader inflammatory signal transduction pathway. Within these pathways it relates to the COX enzymes specifically COX-2 which converts arachidonic acid into PGH2 the precursor of PGE2. This metabolic pathway highlights the interplay between PGE2 and other prostanoids impacting inflammatory responses and homeostatic processes.

PGE2 has associations with conditions such as arthritis and cancer. Elevated levels of PGE2 are often found in inflamed tissues and have been linked to the pathogenesis of rheumatoid arthritis by promoting inflammation and pain. In cancer PGE2 can encourage tumoral growth by modulating the immune response and promoting angiogenesis. In these contexts the interactions of PGE2 with enzymes like COX-2 and cytokines such as tumor necrosis factor (TNF) further connect its roles to disease progression and potential therapeutic targets.
style
left-column

Product protocols

style
left-column

Target data

See full target information Prostaglandin E2
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com