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AB316906

Prostaglandin E2 ELISA Kit - Intracellular

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Prostaglandin E2 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Prostaglandin E2 with a sensitivity of 6.88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

PGE2, Dinoprostone, Prostin E2

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Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)

Example of Prostaglandin E2 standard curve. OD (450 nm) values (mean +/- SD) are graphed.

Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)

Recombinant Prostaglandin E2 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent PTR. Undiluted samples are 0.5 mg/mL Hela cell extract and Jurkat cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)
  • cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Intracellular (AB316906)

Interpolated concentration of native Prostaglandin E2 was measured in duplicate at different sample concentrations. Undiluted sample is 1 mg/mL Human Placenta Extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Competitive

Sensitivity

= 6.88 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Prostaglandin E2 SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Prostaglandin E2 in Cell Lysate and Tissue Extract. Quantitate Prostaglandin E2 with 6.88 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.3" } ]
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Recovery

[ { "sample": "HeLa Extract", "range": "98 - 107 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "93 - 96 %", "average": "= 95" }, { "sample": "Cell Lysate", "range": "102 - 120 %", "average": "= 111" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab316906-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB316906-CMP09" }, { "size":"10 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316906-CMP02" }, { "size":"10 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316906-CMP01" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB316906-CMP08" }, { "size":"10 x 2 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316906-CMP03" }, { "size":"10 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316906-CMP04" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316906-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316906-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316906-CMP10" }, { "size":"1 x 10 Unit", "name":"Plate Seals", "number":"AB316906-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316906-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316906-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316906-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316906-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316906-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316906-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316906-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316906-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316906-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316906-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316906-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316906-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E2 (PGE2) also known as dinoprostone is a lipid compound with a molecular weight of approximately 352.47 g/mol. This compound is synthesized in various tissues like the kidneys lungs and brain primarily by the enzymatic action of cyclooxygenase (COX) on arachidonic acid. As a member of the prostaglandin family PGE2 functions as an important signaling molecule and mediator of inflammation. Researchers often use PGE2 ELISA kits and assays including those specific for mouse PGE2 to measure its levels accurately in biological samples.
Biological function summary

As a potent mediator PGE2 plays roles in inflammation fever induction and regulation of vascular tone. It exerts its effects through specific G-protein-coupled receptors (EP1 EP2 EP3 and EP4) on the surface of target cells. Although not part of a strict protein complex PGE2 works in conjunction with other inflammatory mediators including cytokines and other prostaglandins. It influences cellular activities by modulating the intracellular cyclic AMP levels and calcium fluxes.

Pathways

PGE2 is an important component of the prostaglandin biosynthesis pathway and the broader inflammatory signal transduction pathway. Within these pathways it relates to the COX enzymes specifically COX-2 which converts arachidonic acid into PGH2 the precursor of PGE2. This metabolic pathway highlights the interplay between PGE2 and other prostanoids impacting inflammatory responses and homeostatic processes.

PGE2 has associations with conditions such as arthritis and cancer. Elevated levels of PGE2 are often found in inflamed tissues and have been linked to the pathogenesis of rheumatoid arthritis by promoting inflammation and pain. In cancer PGE2 can encourage tumoral growth by modulating the immune response and promoting angiogenesis. In these contexts the interactions of PGE2 with enzymes like COX-2 and cytokines such as tumor necrosis factor (TNF) further connect its roles to disease progression and potential therapeutic targets.
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Product protocols

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Target data

See full target information Prostaglandin E2
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websiteProtocolBooklet
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