JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273154

Protein A Detection Elisa Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Protein A Detection Elisa Kit is a ELISA for the measurement of Protein A Detection in in samples.

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

1 Images
ELISA - Protein A Detection Elisa Kit (AB273154)
  • ELISA

Supplier Data

ELISA - Protein A Detection Elisa Kit (AB273154)

Protein A Detection Elisa Kit Standard curve.

Example standard curve.

Key facts

Detection method

Colorimetric

Results type

Quantitative

Range

64 - 4000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB215542

Protein A ELISA Kit

SimpleStep

Sandwich ELISA - Protein A ELISA Kit (AB215542)

  • 0
  • 0
Assay Kits

AB133036

Protein A ELISA Kit

Sandwich ELISA - Protein A ELISA Kit (AB133036)

  • 0
  • 0
Assay Kits

AB65393

LDH Assay Kit (Cytotoxicity)

Biochemical assay - LDH Assay Kit (Cytotoxicity) (AB65393)

  • 0
  • 0
Proteins & Peptides

AB52953

Recombinant Staphylococcus aureus Protein A

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Protein A Detection Elisa Kit (ab273154) provides a quick and simple method to estimate the contamination by protein A from Staphylococcus aureus in a solution of antibodies.

The kit includes ready-to-use reagents necessary to analyze 89 samples in 80 minutes.

Capture antibodies coated on the wells, bind the protein A present in the sample and form complexes that are revealed by an anti-Protein A peroxidase conjugated detector antibody. After washing to remove any non-specific binding, the ready-to-use substrate solution is added to microwells and color develops proportionally to the amount of protein A in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab273154-10x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Protein A standard 125 pg/ml", "number":"AB273154-CMP08" }, { "size":"1 x 5 mL", "name":"Protein A standard 62 pg/ml", "number":"AB273154-CMP07" }, { "size":"1 x 5 mL", "name":"Protein A standard 0.0 pg/ml", "number":"AB273154-CMP06" }, { "size":"1 x 5 mL", "name":"Protein A standard 500 pg/ml", "number":"AB273154-CMP09" }, { "size":"1 x 120 mL", "name":"Stop solution", "number":"AB273154-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Substrate", "number":"AB273154-CMP01" }, { "size":"10 x 96 Test", "name":"Pre-coated microwells strips", "number":"AB273154-CMP04" }, { "size":"1 x 120 mL", "name":"Protein A Dissociation Buffer", "number":"AB273154-CMP05" }, { "size":"1 x 500 mL", "name":"Sample Diluent", "number":"AB273154-CMP02" }, { "size":"1 x 120 mL", "name":"Detection antibody", "number":"AB273154-CMP13" }, { "size":"1 x 5 mL", "name":"Protein A standard 4000 pg/ml", "number":"AB273154-CMP12" }, { "size":"1 x 5 mL", "name":"Protein A standard 2000 pg/ml", "number":"AB273154-CMP11" }, { "size":"1 x 5 mL", "name":"Protein A standard 1000 pg/ml", "number":"AB273154-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273154-1x96Tests", "assetComponentDetails": [ { "size":"1 x 0.6 mL", "name":"Protein A standard 125 pg/ml", "number":"AB273154-CMP08", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 62 pg/ml", "number":"AB273154-CMP07", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 0.0 pg/ml", "number":"AB273154-CMP06", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 500 pg/ml", "number":"AB273154-CMP09", "productcode":"" }, { "size":"1 x 96 Test", "name":"Pre-coated microwells strips", "number":"AB273154-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop solution", "number":"AB273154-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent", "number":"AB273154-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB273154-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Protein A Dissociation Buffer", "number":"AB273154-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Detection antibody", "number":"AB273154-CMP13", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 4000 pg/ml", "number":"AB273154-CMP12", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 2000 pg/ml", "number":"AB273154-CMP11", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Protein A standard 1000 pg/ml", "number":"AB273154-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com