RAB10 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of RAB10 in Tissue Extracts, Cell Lysate samples.
Colorimetric
Tissue Extracts, Cell Lysate
Sandwich (quantitative)
Mouse, Rat, Human
0.39 - 25 ng/mL
1h 30m
= 0.112 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21248164). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed:21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed:16641372). In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, it regulates transport from the Golgi to the basolateral membrane (PubMed:16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed:23263280). Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis (PubMed:30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed:30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220).(Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.
Ras-related protein Rab-10, RAB10
RAB10 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of RAB10 in Tissue Extracts, Cell Lysate samples.
Colorimetric
Tissue Extracts, Cell Lysate
Sandwich (quantitative)
Mouse, Rat, Human
0.39 - 25 ng/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 0.112 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Cell Extract | n 8 | mean - | SD - | C.V. 7.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. 8.2 |
Sample type | Average % | Range |
---|---|---|
Sample type Tissue Extracts | Average % = 122 | Range 115 - 127 % |
Sample type Cell Lysate | Average % = 109 | Range 106 - 114 % |
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RAB10 ELISA Kit (ab255717) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RAB10 protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate RAB10 with 0.112 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering, and fusion. Rabs are highly conserved across species; RAB10, also known as Ras-related protein Rab-10, is 100% identical on amino acids level between human and mouse, and it is 97% identical to rat RAB10.
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This supplementary information is collated from multiple sources and compiled automatically.
RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.
RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.
RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.
RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.
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Example of species RAB10 standard curve in 1X Cell Extraction Buffer PTR.
The RAB10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native RAB10 in SH-SY5Y cell extract, C2C12 cell extract, H9c2 cell extract, and human brain tissue extract based on a 250 μg/mL extract load.
The concentrations of RAB10 were measured in duplicate and interpolated from the RAB10 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RAB10 concentration was determined to be 8.650 ng/mL in SH-SY5Y cell extract, 7.369 ng/mL in C2C12 cell extract, 8.645 ng/mL in H9c2 cell extract, and 16.34 ng/mL in human brain tissue extract.
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